PMC:7075884 / 30287-31235 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1091","span":{"begin":861,"end":868},"obj":"Gene"},{"id":"1092","span":{"begin":279,"end":284},"obj":"Species"},{"id":"1093","span":{"begin":377,"end":390},"obj":"Species"},{"id":"1094","span":{"begin":652,"end":657},"obj":"Species"},{"id":"1095","span":{"begin":99,"end":131},"obj":"Chemical"},{"id":"1096","span":{"begin":133,"end":137},"obj":"Chemical"},{"id":"1097","span":{"begin":247,"end":251},"obj":"Chemical"},{"id":"1098","span":{"begin":501,"end":505},"obj":"Chemical"},{"id":"1099","span":{"begin":507,"end":512},"obj":"Chemical"},{"id":"1100","span":{"begin":539,"end":550},"obj":"Chemical"},{"id":"1101","span":{"begin":561,"end":571},"obj":"Chemical"},{"id":"1102","span":{"begin":572,"end":584},"obj":"Chemical"},{"id":"1103","span":{"begin":596,"end":601},"obj":"Chemical"},{"id":"1104","span":{"begin":626,"end":629},"obj":"Chemical"},{"id":"1105","span":{"begin":775,"end":788},"obj":"Chemical"},{"id":"1106","span":{"begin":814,"end":824},"obj":"Chemical"},{"id":"1107","span":{"begin":835,"end":849},"obj":"Chemical"},{"id":"1108","span":{"begin":883,"end":894},"obj":"Chemical"},{"id":"1109","span":{"begin":0,"end":4},"obj":"CellLine"},{"id":"1110","span":{"begin":186,"end":193},"obj":"CellLine"},{"id":"1111","span":{"begin":198,"end":202},"obj":"CellLine"}],"attributes":[{"id":"A1091","pred":"tao:has_database_id","subj":"1091","obj":"Gene:3630"},{"id":"A1092","pred":"tao:has_database_id","subj":"1092","obj":"Tax:10298"},{"id":"A1093","pred":"tao:has_database_id","subj":"1093","obj":"Tax:10298"},{"id":"A1094","pred":"tao:has_database_id","subj":"1094","obj":"Tax:9606"},{"id":"A1100","pred":"tao:has_database_id","subj":"1100","obj":"MESH:D005973"},{"id":"A1101","pred":"tao:has_database_id","subj":"1101","obj":"MESH:D010406"},{"id":"A1102","pred":"tao:has_database_id","subj":"1102","obj":"MESH:D013307"},{"id":"A1103","pred":"tao:has_database_id","subj":"1103","obj":"MESH:D006531"},{"id":"A1104","pred":"tao:has_database_id","subj":"1104","obj":"MESH:D002245"},{"id":"A1105","pred":"tao:has_database_id","subj":"1105","obj":"MESH:D001205"},{"id":"A1106","pred":"tao:has_database_id","subj":"1106","obj":"MESH:D005839"},{"id":"A1107","pred":"tao:has_database_id","subj":"1107","obj":"MESH:D000666"},{"id":"A1108","pred":"tao:has_database_id","subj":"1108","obj":"MESH:D005973"},{"id":"A1109","pred":"tao:has_database_id","subj":"1109","obj":"CVCL:1906"},{"id":"A1110","pred":"tao:has_database_id","subj":"1110","obj":"CVCL:0063"},{"id":"A1111","pred":"tao:has_database_id","subj":"1111","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T242","span":{"begin":5,"end":10},"obj":"Body_part"},{"id":"T243","span":{"begin":173,"end":178},"obj":"Body_part"},{"id":"T244","span":{"begin":204,"end":209},"obj":"Body_part"},{"id":"T245","span":{"begin":446,"end":451},"obj":"Body_part"},{"id":"T246","span":{"begin":541,"end":550},"obj":"Body_part"},{"id":"T247","span":{"begin":658,"end":668},"obj":"Body_part"},{"id":"T248","span":{"begin":701,"end":710},"obj":"Body_part"},{"id":"T249","span":{"begin":861,"end":868},"obj":"Body_part"},{"id":"T250","span":{"begin":885,"end":894},"obj":"Body_part"}],"attributes":[{"id":"A242","pred":"fma_id","subj":"T242","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A243","pred":"fma_id","subj":"T243","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A244","pred":"fma_id","subj":"T244","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A245","pred":"fma_id","subj":"T245","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A246","pred":"fma_id","subj":"T246","obj":"http://purl.org/sig/ont/fma/fma82752"},{"id":"A247","pred":"fma_id","subj":"T247","obj":"http://purl.org/sig/ont/fma/fma54537"},{"id":"A248","pred":"fma_id","subj":"T248","obj":"http://purl.org/sig/ont/fma/fma54537"},{"id":"A249","pred":"fma_id","subj":"T249","obj":"http://purl.org/sig/ont/fma/fma83365"},{"id":"A250","pred":"fma_id","subj":"T250","obj":"http://purl.org/sig/ont/fma/fma82752"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T11","span":{"begin":173,"end":178},"obj":"Body_part"}],"attributes":[{"id":"A11","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T76","span":{"begin":726,"end":729},"obj":"Disease"}],"attributes":[{"id":"A76","pred":"mondo_id","subj":"T76","obj":"http://purl.obolibrary.org/obo/MONDO_0011096"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T462","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/CLO_0003707"},{"id":"T463","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/CLO_0050927"},{"id":"T464","span":{"begin":5,"end":10},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T465","span":{"begin":80,"end":82},"obj":"http://purl.obolibrary.org/obo/CLO_0007622"},{"id":"T466","span":{"begin":198,"end":202},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T467","span":{"begin":198,"end":202},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T468","span":{"begin":198,"end":202},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T469","span":{"begin":204,"end":209},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T470","span":{"begin":295,"end":296},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T471","span":{"begin":373,"end":376},"obj":"http://purl.obolibrary.org/obo/CLO_0053478"},{"id":"T472","span":{"begin":400,"end":401},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T473","span":{"begin":441,"end":445},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T474","span":{"begin":441,"end":445},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T475","span":{"begin":446,"end":451},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T476","span":{"begin":652,"end":657},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T477","span":{"begin":658,"end":668},"obj":"http://purl.obolibrary.org/obo/CL_0000127"},{"id":"T478","span":{"begin":701,"end":710},"obj":"http://purl.obolibrary.org/obo/CL_0000127"},{"id":"T479","span":{"begin":848,"end":849},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T480","span":{"begin":861,"end":868},"obj":"http://purl.obolibrary.org/obo/PR_000009054"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T768","span":{"begin":80,"end":82},"obj":"Chemical"},{"id":"T769","span":{"begin":539,"end":550},"obj":"Chemical"},{"id":"T772","span":{"begin":541,"end":550},"obj":"Chemical"},{"id":"T773","span":{"begin":561,"end":571},"obj":"Chemical"},{"id":"T775","span":{"begin":572,"end":584},"obj":"Chemical"},{"id":"T777","span":{"begin":596,"end":601},"obj":"Chemical"},{"id":"T778","span":{"begin":602,"end":608},"obj":"Chemical"},{"id":"T779","span":{"begin":626,"end":629},"obj":"Chemical"},{"id":"T780","span":{"begin":775,"end":788},"obj":"Chemical"},{"id":"T782","span":{"begin":784,"end":788},"obj":"Chemical"},{"id":"T783","span":{"begin":835,"end":849},"obj":"Chemical"},{"id":"T784","span":{"begin":861,"end":868},"obj":"Chemical"},{"id":"T785","span":{"begin":883,"end":894},"obj":"Chemical"},{"id":"T788","span":{"begin":885,"end":894},"obj":"Chemical"}],"attributes":[{"id":"A768","pred":"chebi_id","subj":"T768","obj":"http://purl.obolibrary.org/obo/CHEBI_74699"},{"id":"A769","pred":"chebi_id","subj":"T769","obj":"http://purl.obolibrary.org/obo/CHEBI_18050"},{"id":"A770","pred":"chebi_id","subj":"T769","obj":"http://purl.obolibrary.org/obo/CHEBI_30011"},{"id":"A771","pred":"chebi_id","subj":"T769","obj":"http://purl.obolibrary.org/obo/CHEBI_58359"},{"id":"A772","pred":"chebi_id","subj":"T772","obj":"http://purl.obolibrary.org/obo/CHEBI_28300"},{"id":"A773","pred":"chebi_id","subj":"T773","obj":"http://purl.obolibrary.org/obo/CHEBI_17334"},{"id":"A774","pred":"chebi_id","subj":"T773","obj":"http://purl.obolibrary.org/obo/CHEBI_51356"},{"id":"A775","pred":"chebi_id","subj":"T775","obj":"http://purl.obolibrary.org/obo/CHEBI_17076"},{"id":"A776","pred":"chebi_id","subj":"T775","obj":"http://purl.obolibrary.org/obo/CHEBI_58007"},{"id":"A777","pred":"chebi_id","subj":"T777","obj":"http://purl.obolibrary.org/obo/CHEBI_42334"},{"id":"A778","pred":"chebi_id","subj":"T778","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A779","pred":"chebi_id","subj":"T779","obj":"http://purl.obolibrary.org/obo/CHEBI_16526"},{"id":"A780","pred":"chebi_id","subj":"T780","obj":"http://purl.obolibrary.org/obo/CHEBI_22652"},{"id":"A781","pred":"chebi_id","subj":"T780","obj":"http://purl.obolibrary.org/obo/CHEBI_29073"},{"id":"A782","pred":"chebi_id","subj":"T782","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"},{"id":"A783","pred":"chebi_id","subj":"T783","obj":"http://purl.obolibrary.org/obo/CHEBI_2682"},{"id":"A784","pred":"chebi_id","subj":"T784","obj":"http://purl.obolibrary.org/obo/CHEBI_145810"},{"id":"A785","pred":"chebi_id","subj":"T785","obj":"http://purl.obolibrary.org/obo/CHEBI_18050"},{"id":"A786","pred":"chebi_id","subj":"T785","obj":"http://purl.obolibrary.org/obo/CHEBI_30011"},{"id":"A787","pred":"chebi_id","subj":"T785","obj":"http://purl.obolibrary.org/obo/CHEBI_58359"},{"id":"A788","pred":"chebi_id","subj":"T788","obj":"http://purl.obolibrary.org/obo/CHEBI_28300"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T140","span":{"begin":711,"end":717},"obj":"http://purl.obolibrary.org/obo/GO_0040007"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"LitCovid-sentences","denotations":[{"id":"T216","span":{"begin":0,"end":185},"obj":"Sentence"},{"id":"T217","span":{"begin":186,"end":278},"obj":"Sentence"},{"id":"T218","span":{"begin":279,"end":372},"obj":"Sentence"},{"id":"T219","span":{"begin":373,"end":440},"obj":"Sentence"},{"id":"T220","span":{"begin":441,"end":630},"obj":"Sentence"},{"id":"T221","span":{"begin":631,"end":895},"obj":"Sentence"},{"id":"T222","span":{"begin":896,"end":948},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}

{"project":"2_test","denotations":[{"id":"32179788-4300104-138463445","span":{"begin":369,"end":371},"obj":"4300104"},{"id":"32179788-10196307-138463446","span":{"begin":437,"end":439},"obj":"10196307"}],"text":"HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS). HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS. HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28. GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29. Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2. Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine. Early passages (1–4) were used in these experiments."}