PMC:7075884 / 14730-16162 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7075884","sourcedb":"PMC","sourceid":"7075884","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7075884","text":"GA inhibits a non-enveloped adenovirus\nTo assess the effect of GA on non-enveloped virus, we also tested its antiviral activity on adenovirus, which is internalized by endocytosis. Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells then were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) or GFP-HSV-1 virus, at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. The inoculated Vero cells were evaluated by fluorescence microscopy (Fig. 4). The results showed significant HSV-1 inhibition, as well as inhibition of entry of the adenovirus.\nFigure 4 GA inhibits non-enveloped human adenovirus. (A) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells were infected with GFP-HSV-1 virus at an MOI of 0.5 in medium containing 10 µM GA C15:1 for 24 hours. Infection was evaluated by light (lower panels) and fluorescence (upper panels) microscopy. (B) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM of GA C15:1 or DMSO vehicle. The cells were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. Inhibition of entry was evaluated by light (lower panels) and fluorescence (upper panels) 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