PMC:7068163 / 14309-15070 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"189","span":{"begin":152,"end":153},"obj":"Gene"},{"id":"190","span":{"begin":4,"end":14},"obj":"Species"},{"id":"191","span":{"begin":414,"end":425},"obj":"Species"},{"id":"192","span":{"begin":444,"end":447},"obj":"Species"},{"id":"193","span":{"begin":461,"end":471},"obj":"Species"}],"attributes":[{"id":"A189","pred":"tao:has_database_id","subj":"189","obj":"Gene:43740570"},{"id":"A190","pred":"tao:has_database_id","subj":"190","obj":"Tax:2697049"},{"id":"A191","pred":"tao:has_database_id","subj":"191","obj":"Tax:11118"},{"id":"A192","pred":"tao:has_database_id","subj":"192","obj":"Tax:11118"},{"id":"A193","pred":"tao:has_database_id","subj":"193","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T50","span":{"begin":154,"end":158},"obj":"Body_part"}],"attributes":[{"id":"A50","pred":"fma_id","subj":"T50","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T46","span":{"begin":4,"end":12},"obj":"Disease"},{"id":"T47","span":{"begin":461,"end":469},"obj":"Disease"}],"attributes":[{"id":"A46","pred":"mondo_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T142","span":{"begin":154,"end":158},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T143","span":{"begin":238,"end":244},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T144","span":{"begin":310,"end":317},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T145","span":{"begin":442,"end":443},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T146","span":{"begin":487,"end":488},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"LitCovid-sentences","denotations":[{"id":"T111","span":{"begin":0,"end":193},"obj":"Sentence"},{"id":"T112","span":{"begin":194,"end":501},"obj":"Sentence"},{"id":"T113","span":{"begin":502,"end":667},"obj":"Sentence"},{"id":"T114","span":{"begin":668,"end":761},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"2_test","denotations":[{"id":"32156330-16274752-29325827","span":{"begin":41,"end":42},"obj":"16274752"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}

{"project":"MyTest","denotations":[{"id":"32156330-16274752-29325827","span":{"begin":41,"end":42},"obj":"16274752"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"The SARS-CoV-2 assay from Corman et al. [7] could be used rapidly with the published SuperScript III system, but further optimisation especially of the E gene assay may enhance the sensitivity. The RealStar kit outperformed the two other tested one-step PCR systems in sensitivity and by absence of unspecific signals with the used primer set and the RealStar workflow enhanced laboratory efficiency combining two coronavirus assays (lineage B CoV and specific SARS-CoV-2) with an IC in a triplex PCR. Overall, fast assay development and publication of protocols by expert laboratories allowed public health laboratories to establish diagnostics quickly and act fast. Assay optimisation will further speed up and improve the management of this ongoing outbreak."}