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PMC:7068163 / 13088-14296 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
178 640-641 Gene denotes E Gene:43740570
179 1145-1146 Gene denotes E Gene:43740570
180 992-993 Gene denotes E Gene:43740570
181 540-541 Gene denotes E Gene:43740570
182 405-406 Gene denotes E Gene:43740570
183 150-166 Chemical denotes oligonucleotides MESH:D009841

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T43 292-295 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T44 343-346 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T45 407-411 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T46 542-546 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T47 642-646 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T48 994-998 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T49 1147-1151 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T127 17-18 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T128 41-48 http://purl.obolibrary.org/obo/SO_0000418 denotes signals
T129 225-226 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T130 321-322 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T131 360-361 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T132 379-380 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T133 407-411 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T134 542-546 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T135 642-646 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T136 730-731 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T137 784-791 http://purl.obolibrary.org/obo/SO_0000418 denotes signals
T138 821-828 http://purl.obolibrary.org/obo/SO_0000418 denotes signals
T139 994-998 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T140 1147-1151 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T141 1171-1172 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T16 150-166 Chemical denotes oligonucleotides http://purl.obolibrary.org/obo/CHEBI_7754
T17 185-193 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893
T18 457-465 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893
T19 1011-1016 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T102 0-268 Sentence denotes Contamination as a reason for unspecific signals was ruled out, as stringent prevention measures were taken, e.g. strict separation of working areas: oligonucleotides and PCR mastermix reagents were handled in one room under a PCR hood with specified laboratory coats.
T103 269-335 Sentence denotes Sample preparation and RNA extraction took place in a second room.
T104 336-390 Sentence denotes Sample RNA was added in a third room under a PCR hood.
T105 391-452 Sentence denotes The synthetic E gene control was added last to the mastermix.
T106 453-509 Sentence denotes All reagents were aliquoted and aliquots used once only.
T107 510-792 Sentence denotes Contaminations from synthetic E gene present in primer batches upon delivery can be ruled out as well, although only one batch of E gene primers and probes was used with the QuantiTect and Superscript III setup, as only a certain proportion of samples showed the unspecific signals.
T108 793-959 Sentence denotes Furthermore, the unspecific signals were significantly reduced in the Superscript III setup, which showed that its sensitivity was comparable to the QuantiTect setup.
T109 960-1208 Sentence denotes In addition, the initially used E gene primers and probe were separately used as templates with the RealStar kit and no amplification was observed, whereas the corresponding artificial E gene template delivered a clear S-shaped curve with this kit.