PMC:7068163 / 128-659 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"6","span":{"begin":134,"end":181},"obj":"Species"},{"id":"7","span":{"begin":183,"end":193},"obj":"Species"}],"attributes":[{"id":"A6","pred":"tao:has_database_id","subj":"6","obj":"Tax:2697049"},{"id":"A7","pred":"tao:has_database_id","subj":"7","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T2","span":{"begin":134,"end":181},"obj":"Disease"},{"id":"T3","span":{"begin":134,"end":167},"obj":"Disease"},{"id":"T4","span":{"begin":183,"end":191},"obj":"Disease"}],"attributes":[{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1","span":{"begin":252,"end":253},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T2","span":{"begin":375,"end":376},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well."}

{"project":"LitCovid-sentences","denotations":[{"id":"T3","span":{"begin":0,"end":205},"obj":"Sentence"},{"id":"T4","span":{"begin":206,"end":392},"obj":"Sentence"},{"id":"T5","span":{"begin":393,"end":531},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well."}