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PMC:7048229 / 4341-5229 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T15 0-3 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T16 127-130 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T17 193-196 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T18 210-213 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T19 332-336 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T20 387-390 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T21 691-694 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T36 332-336 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T37 543-546 http://purl.obolibrary.org/obo/CLO_0007169 denotes l 2
T38 543-546 http://purl.obolibrary.org/obo/CLO_0007170 denotes l 2
T39 836-837 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T13 245-251 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T14 438-440 Chemical denotes II http://purl.obolibrary.org/obo/CHEBI_74067
T15 490-492 Chemical denotes Co http://purl.obolibrary.org/obo/CHEBI_27638
T16 682-694 Chemical denotes template RNA http://purl.obolibrary.org/obo/CHEBI_33699

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T46 0-26 Sentence denotes RNA extraction and qRT-PCR
T47 27-126 Sentence denotes Whenever commercial kits were used, manufacturer’s instructions were followed without modification.
T48 127-209 Sentence denotes RNA was extracted from 200 μl of samples with the High Pure Viral RNA Kit (Roche).
T49 210-288 Sentence denotes RNA was eluted in 50 μl of elution buffer and used as the template for RT–PCR.
T50 289-376 Sentence denotes QPCR detection method based on 2019-nCoV S gene can be found in the previous study [5].
T51 377-500 Sentence denotes In brief, RNA extracted from above used in qPCR by HiScript® II One Step qRT-PCR SYBR® Green Kit (Vazyme Biotech Co., Ltd).
T52 501-695 Sentence denotes The 20 μl qPCR reaction mix contained 10 μl 2× One Step SYBR Green Mix, 1 μl One Step SYBR Green Enzyme Mix, 0.4 μl 50 × ROX Reference Dye 1, 0.4 μl of each primer (10 μM) and 2 μl template RNA.
T53 696-735 Sentence denotes Amplification was performed as follows:
T54 736-888 Sentence denotes 50°C for 3 min, 95°C for 30 s followed by 40 cycles consisting of 95°C for 10 s, 60°C for 30 s, and a default melting curve step in an ABI 7500 machine.

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
113 568-571 Gene denotes Mix Gene:83881
114 622-625 Gene denotes ROX Gene:4335
115 605-608 Gene denotes Mix Gene:83881
116 525-528 Gene denotes mix Gene:83881
117 320-329 Species denotes 2019-nCoV Tax:2697049