PMC:7045880 / 7868-8750 JSONTXT

Annnotations TAB JSON ListView MergeView

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7045880","sourcedb":"PMC","sourceid":"7045880","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7045880","text":"1. Virus isolation from Vero cells\nFollowing inoculation of Vero cells with the nasopharyngeal and oropharyngeal samples, they were observed at 24-hour intervals, and the cytopathic effects were observed from 3 days after inoculation (Figure 1). The inoculated cells were harvested on the 4th day when more than 80% of the cells exhibited cytopathic effects. Virus replication was confirmed using real-time RT-PCR with RNA extracted from the cell culture medium. The Ct values were 14.40 and 18.26 for the nasopharyngeal and oropharyngeal samples, respectively, which were lower than the cycle threshold (Ct) values of 20.85 and 21.85 in the pre-inoculated samples. The number of virus copies in the samples before inoculation was 7.6 × 108 and 3.9 × 108 copy/mL, respectively, and increased by 10–70-fold to 5.4 × 1010 and 4.2 × 109, respectively, in the cell culture supernatants.","divisions":[{"label":"title","span":{"begin":0,"end":34}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T35","span":{"begin":29,"end":34},"obj":"Body_part"},{"id":"T36","span":{"begin":65,"end":70},"obj":"Body_part"},{"id":"T37","span":{"begin":261,"end":266},"obj":"Body_part"},{"id":"T38","span":{"begin":323,"end":328},"obj":"Body_part"},{"id":"T39","span":{"begin":419,"end":422},"obj":"Body_part"},{"id":"T40","span":{"begin":442,"end":446},"obj":"Body_part"},{"id":"T41","span":{"begin":856,"end":860},"obj":"Body_part"}],"attributes":[{"id":"A35","pred":"fma_id","subj":"T35","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A36","pred":"fma_id","subj":"T36","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A37","pred":"fma_id","subj":"T37","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A38","pred":"fma_id","subj":"T38","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A39","pred":"fma_id","subj":"T39","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A40","pred":"fma_id","subj":"T40","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A41","pred":"fma_id","subj":"T41","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T35","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T37","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T38","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T39","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T40","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T41","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T71","span":{"begin":3,"end":8},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T72","span":{"begin":24,"end":28},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T73","span":{"begin":24,"end":28},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T74","span":{"begin":29,"end":34},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T75","span":{"begin":60,"end":64},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T76","span":{"begin":60,"end":64},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T77","span":{"begin":65,"end":70},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T78","span":{"begin":261,"end":266},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T79","span":{"begin":323,"end":328},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T80","span":{"begin":359,"end":364},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T81","span":{"begin":442,"end":446},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T82","span":{"begin":680,"end":685},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T83","span":{"begin":856,"end":860},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"attributes":[{"subj":"T71","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T73","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T74","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T75","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T76","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T77","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T78","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T79","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T80","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T81","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T82","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T83","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T74","span":{"begin":0,"end":2},"obj":"Sentence"},{"id":"T75","span":{"begin":3,"end":34},"obj":"Sentence"},{"id":"T76","span":{"begin":35,"end":245},"obj":"Sentence"},{"id":"T77","span":{"begin":246,"end":358},"obj":"Sentence"},{"id":"T78","span":{"begin":359,"end":462},"obj":"Sentence"},{"id":"T79","span":{"begin":463,"end":665},"obj":"Sentence"},{"id":"T80","span":{"begin":666,"end":882},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T74","pred":"source","obj":"LitCovid-sentences"},{"subj":"T75","pred":"source","obj":"LitCovid-sentences"},{"subj":"T76","pred":"source","obj":"LitCovid-sentences"},{"subj":"T77","pred":"source","obj":"LitCovid-sentences"},{"subj":"T78","pred":"source","obj":"LitCovid-sentences"},{"subj":"T79","pred":"source","obj":"LitCovid-sentences"},{"subj":"T80","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93ecb0","default":true},{"id":"LitCovid-PD-CLO","color":"#ec9693"},{"id":"LitCovid-sentences","color":"#93a9ec"}]}]}}