PMC:7045880 / 5803-6310 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"143","span":{"begin":315,"end":325},"obj":"Species"}],"attributes":[{"id":"A143","pred":"tao:has_database_id","subj":"143","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T28","span":{"begin":55,"end":58},"obj":"Body_part"},{"id":"T29","span":{"begin":87,"end":91},"obj":"Body_part"},{"id":"T30","span":{"begin":326,"end":329},"obj":"Body_part"},{"id":"T31","span":{"begin":500,"end":506},"obj":"Body_part"}],"attributes":[{"id":"A28","pred":"fma_id","subj":"T28","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A29","pred":"fma_id","subj":"T29","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A30","pred":"fma_id","subj":"T30","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma84116"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T28","span":{"begin":315,"end":323},"obj":"Disease"}],"attributes":[{"id":"A28","pred":"mondo_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T55","span":{"begin":78,"end":91},"obj":"http://purl.obolibrary.org/obo/CL_0000010"},{"id":"T56","span":{"begin":112,"end":117},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T57","span":{"begin":134,"end":135},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T58","span":{"begin":226,"end":231},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T59","span":{"begin":486,"end":487},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T27","span":{"begin":326,"end":329},"obj":"Chemical"},{"id":"T28","span":{"begin":402,"end":404},"obj":"Chemical"}],"attributes":[{"id":"A27","pred":"chebi_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A28","pred":"chebi_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A29","pred":"chebi_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A30","pred":"chebi_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T8","span":{"begin":14,"end":27},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T9","span":{"begin":14,"end":27},"obj":"http://purl.obolibrary.org/obo/GO_0003899"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}

{"project":"LitCovid-sentences","denotations":[{"id":"T55","span":{"begin":0,"end":133},"obj":"Sentence"},{"id":"T56","span":{"begin":134,"end":339},"obj":"Sentence"},{"id":"T57","span":{"begin":340,"end":405},"obj":"Sentence"},{"id":"T58","span":{"begin":406,"end":507},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. The prepared library was purified and analyzed with Miseq 150 PE. De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome."}