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PMC:7045880 / 3721-4918 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
119 148-156 Species denotes SARS-CoV Tax:694009

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T17 129-132 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T18 198-201 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T19 212-215 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T20 227-231 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T21 444-448 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T22 667-670 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T25 148-156 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T35 78-79 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T36 227-231 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T37 444-448 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T38 617-618 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T39 1159-1169 http://purl.obolibrary.org/obo/OBI_0000968 denotes instrument

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T9 173-178 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406
T10 377-382 Chemical denotes 5-FAM http://purl.obolibrary.org/obo/CHEBI_51617
T11 417-422 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406
T12 595-600 Chemical denotes 5-FAM http://purl.obolibrary.org/obo/CHEBI_51617
T13 696-702 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T14 816-823 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T15 890-895 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T4 906-927 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T5 914-927 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T6 1006-1019 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T7 1006-1019 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T39 0-157 Sentence denotes The optimal concentration of primers and probes, which were synthesized using a published sequence [12], was determined with the RNA transcripts of SARS-CoV.
T40 158-247 Sentence denotes The primer and probe sequences used for RNA-dependent RNA polymerase gene detection were:
T41 248-464 Sentence denotes 5′-GTGARATGGTCATGTGTGGCGG-3′ (Forward), 5′-CARATGTTAAASACACTATTAGCATA-3′ (Reverse) and 5′-CAGGTGGAACCTCATCAGGAGATGC-3′ (Probe in 5-FAM/3′-BHQ format) and the primer and probe sequences used for E gene detection were:
T42 465-616 Sentence denotes 5′-ACAGGTACGTTAATAGTTAATAGCGT-3′ (Forward), 5′-ATATTGCAGCAGTACGCACACA-3′ (Reverse) and 5′-ACACTAGCCATCCTTACTGCGCTTCG-3′ (Probe in 5-FAM/3′-BHQ format).
T43 617-905 Sentence denotes A 25-μL reaction was setup that contained 5 μL of RNA, 12.5 μL of 2 × reaction buffer provided with the Agpath IDTM 1 step RT-PCR system (Thermo Fisher Scientific, Waltham, USA), 1 μL of 25 × enzyme mixture, 1 μL of forward and reverse primers at 10 pM, and 0.5 μL of each probe at 10 pM.
T44 906-1043 Sentence denotes Reverse transcription was performed at 50°C for 30 minutes, followed by inactivation of the reverse transcriptase at 95°C for 10 minutes.
T45 1044-1197 Sentence denotes PCR amplification was performed with 40 cycles at 95°C for 15 seconds and 60°C for 1 minute using an ABI 7500 Fast instrument (Thermo Fisher Scientific).