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PMC:7045880 / 3701-4918 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
119 168-176 Species denotes SARS-CoV Tax:694009

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T17 149-152 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T18 218-221 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T19 232-235 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T20 247-251 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T21 464-468 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T22 687-690 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T25 168-176 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T35 98-99 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T36 247-251 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T37 464-468 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T38 637-638 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T39 1179-1189 http://purl.obolibrary.org/obo/OBI_0000968 denotes instrument

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T9 193-198 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406
T10 397-402 Chemical denotes 5-FAM http://purl.obolibrary.org/obo/CHEBI_51617
T11 437-442 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406
T12 615-620 Chemical denotes 5-FAM http://purl.obolibrary.org/obo/CHEBI_51617
T13 716-722 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T14 836-843 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T15 910-915 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T4 926-947 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T5 934-947 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T6 1026-1039 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T7 1026-1039 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T37 0-2 Sentence denotes 2.
T38 3-19 Sentence denotes Real-time RT-PCR
T39 20-177 Sentence denotes The optimal concentration of primers and probes, which were synthesized using a published sequence [12], was determined with the RNA transcripts of SARS-CoV.
T40 178-267 Sentence denotes The primer and probe sequences used for RNA-dependent RNA polymerase gene detection were:
T41 268-484 Sentence denotes 5′-GTGARATGGTCATGTGTGGCGG-3′ (Forward), 5′-CARATGTTAAASACACTATTAGCATA-3′ (Reverse) and 5′-CAGGTGGAACCTCATCAGGAGATGC-3′ (Probe in 5-FAM/3′-BHQ format) and the primer and probe sequences used for E gene detection were:
T42 485-636 Sentence denotes 5′-ACAGGTACGTTAATAGTTAATAGCGT-3′ (Forward), 5′-ATATTGCAGCAGTACGCACACA-3′ (Reverse) and 5′-ACACTAGCCATCCTTACTGCGCTTCG-3′ (Probe in 5-FAM/3′-BHQ format).
T43 637-925 Sentence denotes A 25-μL reaction was setup that contained 5 μL of RNA, 12.5 μL of 2 × reaction buffer provided with the Agpath IDTM 1 step RT-PCR system (Thermo Fisher Scientific, Waltham, USA), 1 μL of 25 × enzyme mixture, 1 μL of forward and reverse primers at 10 pM, and 0.5 μL of each probe at 10 pM.
T44 926-1063 Sentence denotes Reverse transcription was performed at 50°C for 30 minutes, followed by inactivation of the reverse transcriptase at 95°C for 10 minutes.
T45 1064-1217 Sentence denotes PCR amplification was performed with 40 cycles at 95°C for 15 seconds and 60°C for 1 minute using an ABI 7500 Fast instrument (Thermo Fisher Scientific).