PMC:7025468 / 6741-7565 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7025468","sourcedb":"PMC","sourceid":"7025468","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7025468","text":"Primary IRAK1 and TRAF6 antibodies were obtained from Cell Signaling Technology (Danvers, MA). GAPDH was used as a loading control (Santa Cruz Biotechnology, Dallas, TX). Western Blotting was performed as described previously (36). Briefly, cells were collected and lysed, and protein was separated using NuPAGE pre-cast gels (Life Technologies Corp., Carlsbad, CA). Gels were transferred via semi-dry electrotransfer to 0.45 μm nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA), and probed with designated primary antibodies. Blots were then probed with LI-COR IRDye secondary antibodies and imaged using LI-COR Odyssey CLX according to the manufacturer's instructions (LI-COR, Lincoln, Nebraska). Analysis and relative quantification of gel bands was carried out using ImageJ software (NIH, Bethesda, MD) (37).","tracks":[{"project":"TEST0","denotations":[{"id":"32117283-56-62-3683893","span":{"begin":227,"end":229},"obj":"[\"23119100\"]"},{"id":"32117283-109-115-3683894","span":{"begin":820,"end":822},"obj":"[\"22930834\"]"}],"attributes":[{"subj":"32117283-56-62-3683893","pred":"source","obj":"TEST0"},{"subj":"32117283-109-115-3683894","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"32117283-23119100-35221699","span":{"begin":227,"end":229},"obj":"23119100"},{"id":"32117283-22930834-35221700","span":{"begin":820,"end":822},"obj":"22930834"}],"attributes":[{"subj":"32117283-23119100-35221699","pred":"source","obj":"2_test"},{"subj":"32117283-22930834-35221700","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"32117283-23119100-35221699","span":{"begin":227,"end":229},"obj":"23119100"},{"id":"32117283-22930834-35221700","span":{"begin":820,"end":822},"obj":"22930834"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32117283-23119100-35221699","pred":"source","obj":"MyTest"},{"subj":"32117283-22930834-35221700","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#ec93d8","default":true},{"id":"2_test","color":"#93ece6"},{"id":"MyTest","color":"#eccc93"}]}]}}