PMC:7025468 / 14944-15799 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7025468","sourcedb":"PMC","sourceid":"7025468","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7025468","text":"Meth increases miR-146a expression and downregulates of TRAF6. CD4+ T-cells were treated daily with or without Meth. (A) Expression of miR-146a and IL-1β mRNA was determined by RT-qPCR. Fold change was calculated by normalizing the Meth treated cells to untreated cells. Data represent the mean ± SD of 3 independent experiments, and p-values were calculated relative to untreated controls (*p ≤ 0.05, **p ≤ 0.01). (B) Cells treated or untreated with Meth were lysed and protein extracts were analyzed for expression of TRAF6 and IRAK1 by Western Blotting. GAPDH was used as a loading control. Relative band intensity was calculated using ImageJ software. (C) Expression of TRAF6 mRNA was determined by RT-qPCR. Fold change was calculated by normalizing the Meth treated cells to untreated cells. Data represent the mean ± SD of 3 independent experiments.","tracks":[]}