PMC:7019868 / 9497-10575
Annnotations
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
187 | 942-945 | Gene | denotes | Rad | Gene:6236 |
188 | 55-59 | Species | denotes | PEDV | Tax:28295 |
189 | 580-584 | Species | denotes | goat | Tax:9925 |
190 | 590-595 | Species | denotes | mouse | Tax:10090 |
191 | 330-337 | Chemical | denotes | acetone | MESH:D000096 |
192 | 516-541 | Chemical | denotes | phosphate-buffered saline | |
193 | 543-546 | Chemical | denotes | PBS | |
194 | 553-557 | Chemical | denotes | FITC | MESH:D016650 |
195 | 780-809 | Chemical | denotes | 4′,6-diamidino-2-phenylindole | MESH:C007293 |
196 | 811-815 | Chemical | denotes | DAPI | MESH:C007293 |
197 | 185-193 | Disease | denotes | infected | MESH:D007239 |
LitCovid-PD-FMA-UBERON
Id | Subject | Object | Predicate | Lexical cue | fma_id |
---|---|---|---|---|---|
T47 | 132-137 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T48 | 168-173 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T49 | 295-300 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T50 | 398-406 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T51 | 599-607 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T52 | 728-733 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T53 | 925-929 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
LitCovid-PD-MONDO
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T14 | 245-254 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
T15 | 284-293 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
T16 | 609-611 | Disease | denotes | BD | http://purl.obolibrary.org/obo/MONDO_0007191 |
LitCovid-PD-CLO
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T108 | 113-115 | http://purl.obolibrary.org/obo/CLO_0050510 | denotes | 18 |
T109 | 127-131 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
T110 | 127-131 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
T111 | 132-137 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T112 | 168-173 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T113 | 227-228 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T114 | 274-276 | http://purl.obolibrary.org/obo/CLO_0050510 | denotes | 18 |
T115 | 295-300 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T116 | 421-422 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T117 | 473-478 | http://purl.obolibrary.org/obo/CLO_0003744 | denotes | h (h) |
T118 | 580-584 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T119 | 590-595 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
T120 | 658-659 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T121 | 728-733 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T122 | 925-929 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T123 | 1068-1077 | http://purl.obolibrary.org/obo/CL_0000228 | denotes | syncytium |
LitCovid-PD-CHEBI
Id | Subject | Object | Predicate | Lexical cue | chebi_id |
---|---|---|---|---|---|
T35 | 60-68 | Chemical | denotes | antigens | http://purl.obolibrary.org/obo/CHEBI_59132 |
T36 | 330-337 | Chemical | denotes | acetone | http://purl.obolibrary.org/obo/CHEBI_15347 |
T37 | 516-525 | Chemical | denotes | phosphate | http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474 |
T41 | 553-557 | Chemical | denotes | FITC | http://purl.obolibrary.org/obo/CHEBI_37926 |
T42 | 596-598 | Chemical | denotes | IG | http://purl.obolibrary.org/obo/CHEBI_74066 |
T43 | 797-809 | Chemical | denotes | phenylindole | http://purl.obolibrary.org/obo/CHEBI_48559 |
T44 | 811-815 | Chemical | denotes | DAPI | http://purl.obolibrary.org/obo/CHEBI_51231 |
T45 | 835-837 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
T48 | 1057-1063 | Chemical | denotes | nuclei | http://purl.obolibrary.org/obo/CHEBI_33252 |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T69 | 0-117 | Sentence | denotes | Immunofluorescence assay (IFA) was performed to detect PEDV antigens as previously described with modifications [18]. |
T70 | 118-270 | Sentence | denotes | Briefly, Vero cells in 96-well plates (1.75 × 104 cells/well) were infected with the designated P1viruses at a multiplicity of infection (MOI) of 0.005. |
T71 | 271-479 | Sentence | denotes | At 18 h post-infection, cells were fixed with 80% ice-cold acetone, air-dried, and then incubated with an in-house anti-PEDV S antibody, P4B [20], at a dilution of 1:1000 at room temperature (RT) for 1 h (h). |
T72 | 480-692 | Sentence | denotes | After being washed three times with phosphate-buffered saline (PBS), the FITC-conjugated monoclonal goat anti-mouse-IG antibody (BD Pharmingen, San Jose, CA, USA) was applied at a dilution of 1:500 at RT for 1 h. |
T73 | 693-866 | Sentence | denotes | Following the final wash step, the cells were counterstained with mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Abcam, Cambridge, MA, USA) in the dark for 1 min. |
T74 | 867-966 | Sentence | denotes | Images were visualized and captured using ZOE fluorescent cell imager (Bio-Rad, Hercules, CA, USA). |
T75 | 967-1078 | Sentence | denotes | Syncytia analysis were performed concurrently along with IFA by calculating the number of nuclei per syncytium. |
2_test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
31905842-30792462-144353226 | 413-415 | 30792462 | denotes | 20 |