PMC:7019868 / 8576-8894 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"155","span":{"begin":284,"end":288},"obj":"Species"},{"id":"163","span":{"begin":233,"end":258},"obj":"Chemical"},{"id":"164","span":{"begin":260,"end":263},"obj":"Chemical"}],"attributes":[{"id":"A155","pred":"tao:has_database_id","subj":"155","obj":"Tax:28295"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T41","span":{"begin":60,"end":63},"obj":"Body_part"},{"id":"T42","span":{"begin":199,"end":204},"obj":"Body_part"},{"id":"T43","span":{"begin":213,"end":218},"obj":"Body_part"}],"attributes":[{"id":"A41","pred":"fma_id","subj":"T41","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A42","pred":"fma_id","subj":"T42","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A43","pred":"fma_id","subj":"T43","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T95","span":{"begin":82,"end":83},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T96","span":{"begin":199,"end":204},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T97","span":{"begin":208,"end":212},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T98","span":{"begin":208,"end":212},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T99","span":{"begin":213,"end":218},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T26","span":{"begin":233,"end":242},"obj":"Chemical"}],"attributes":[{"id":"A26","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_18367"},{"id":"A27","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_26020"},{"id":"A28","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_35780"},{"id":"A29","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_43474"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T26","span":{"begin":105,"end":118},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}

{"project":"LitCovid-sentences","denotations":[{"id":"T61","span":{"begin":0,"end":318},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts."}