PMC:7019868 / 7688-7955 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"154","span":{"begin":131,"end":138},"obj":"Species"}],"attributes":[{"id":"A154","pred":"tao:has_database_id","subj":"154","obj":"Tax:562"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"However, we split the plasmid B into two fragments because the sequence remained toxic to the One Shot™ TOP10 Chemically Competent E. coli cells (Invitrogen, Carlsbad, USA) despite propagation in LB broth supplemented with 10% SOC medium and being incubated at 30 °C."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T38","span":{"begin":139,"end":144},"obj":"Body_part"}],"attributes":[{"id":"A38","pred":"fma_id","subj":"T38","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"However, we split the plasmid B into two fragments because the sequence remained toxic to the One Shot™ TOP10 Chemically Competent E. coli cells (Invitrogen, Carlsbad, USA) despite propagation in LB broth supplemented with 10% SOC medium and being incubated at 30 °C."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T86","span":{"begin":30,"end":31},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T87","span":{"begin":139,"end":144},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"However, we split the plasmid B into two fragments because the sequence remained toxic to the One Shot™ TOP10 Chemically Competent E. coli cells (Invitrogen, Carlsbad, USA) despite propagation in LB broth supplemented with 10% SOC medium and being incubated at 30 °C."}

{"project":"LitCovid-sentences","denotations":[{"id":"T56","span":{"begin":0,"end":267},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"However, we split the plasmid B into two fragments because the sequence remained toxic to the One Shot™ TOP10 Chemically Competent E. coli cells (Invitrogen, Carlsbad, USA) despite propagation in LB broth supplemented with 10% SOC medium and being incubated at 30 °C."}