PMC:7019868 / 6775-7388 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7019868","sourcedb":"PMC","sourceid":"7019868","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7019868","text":"2.2. Cells and Viruses\nVero C1008 cells (ATCC No. CRL-1586) were maintained in growth medium containing Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine protein (FBS), 250 ng/mL Amphotericin B, 100 U/mL Penicillin and 100 μg/mL Streptomycin. The recombinant viruses (iPEDVPT-P5 and iPEDVPT-P96), and the chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S), were propagated in post-inoculation medium (PI medium) containing DMEM supplemented with 0.3% tryptose phosphate broth (TBP), 0.02% yeast extract, and 10 μg/mL trypsin as described previously 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