PMC:7019868 / 32307-33624 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7019868","sourcedb":"PMC","sourceid":"7019868","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7019868","text":"Figure 1 In vitro characterization of the recombinant iPEDVPT-P5, iPEDVPT-P5-96S, iPEDVPT-P96-5S and iPEDVPT-P96 viruses. (A) Representative immunofluorescence micrographs of porcine epidemic diarrhea virus (PEDV) syncytia in Vero cells stained with anti-PEDV spike antibody at 18 h post-infection (HPI). Note the recombinant viruses carrying spike gene derived from the attenuated iPEDVPT-P96 showed larger syncytia. Scale Bar = 50 μm. (B) The number of nuclei per syncytium was quantified and assessed statistically using Kruskal–Wallis test after immunofluorescence staining. Statistical differences (p \u003c 0.05) among groups were indicated by different letters. (C) Growth kinetics of recombinant viruses in Vero cells after infection at multiplicity of infection (MOI) of 1 and 0.001. Supernatant were collected at indicated time points and the titers were quantified in Vero cells by standard 50% tissue-culture infectious dose (TCID50) assay. (D) Plaque morphologies in Vero cells of the recombinant viruses. The plates were stained with 1% crystal violet solution after 72 HPI. Note that comparing to the iPEDVPT-P96 and iPEDVPT-P5-96S, the plaques produced by iPEDVPT-P5 virus and iPEDVPT-P96-5S were barely visible macroscopically. iP5: iPEDVPT-P5; 96S: iPEDVPT-P5-96S; 5S, iPEDVPT-P96-5S; iP96, 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