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PMC:7019868 / 17459-18897 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
315 92-96 Species denotes PEDV Tax:28295
316 232-236 Species denotes PEDV Tax:28295
317 150-157 Chemical denotes iPEDVPT
318 428-435 Chemical denotes iPEDVPT
319 485-492 Chemical denotes iPEDVPT
320 724-731 Chemical denotes iPEDVPT
321 732-734 Chemical denotes P5 MESH:C016883
322 739-746 Chemical denotes iPEDVPT
323 968-978 Chemical denotes iPEDVPT-P5
328 1133-1137 Species denotes PEDV Tax:28295
329 1224-1230 Species denotes stocks Tax:3724
330 1326-1330 Species denotes PEDV Tax:28295
331 1160-1174 Disease denotes giant syncytia MESH:D005870

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T87 300-304 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T88 545-549 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T89 798-808 Body_part denotes nucleotide http://purl.org/sig/ont/fma/fma82740
T90 1274-1279 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T91 1368-1375 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T176 62-63 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T177 129-130 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T178 143-148 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T179 245-247 http://purl.obolibrary.org/obo/CLO_0001407 denotes 52
T180 300-304 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T181 417-419 http://purl.obolibrary.org/obo/CLO_0001407 denotes 52
T182 457-464 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes viruses
T183 545-549 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T184 571-573 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T185 631-638 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes viruses
T186 776-777 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T187 1049-1050 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T188 1098-1100 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T189 1209-1216 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes viruses
T190 1258-1265 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes viruses
T191 1269-1273 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T192 1269-1273 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T193 1274-1279 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T194 1301-1303 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T102 798-808 Chemical denotes nucleotide http://purl.obolibrary.org/obo/CHEBI_36976
T103 1301-1303 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T104 1368-1375 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T129 0-4 Sentence denotes 3.1.
T130 5-34 Sentence denotes Recovery of recombinant PEDVs
T131 35-272 Sentence denotes We have recently developed a reverse genetic platform of PEDV and have successfully generated a recombinant virus, iPEDVPT-P96, which is phenotypically comparable to its parental attenuated Taiwan PEDV-Pintung 52 strain, PEDVPT-P96 [19].
T132 273-575 Sentence denotes To study the role of the S gene in the attenuation mechanism of PEDVPT-P96, we further generated the recombinant, highly virulent Taiwan PEDVPT 52 strain, iPEDVPT-P5, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) by replacing the complete sequence of the S gene reciprocally (Figure S1).
T133 576-692 Sentence denotes In general, the approach to rescue the new recombinant viruses were virtually the same as published previously [19].
T134 693-928 Sentence denotes However, for the generation of iPEDVPT-P5 and iPEDVPT-P5-96S, we split the plasmid B to two fragments at nucleotide position of 9654 according to Hou et al. [23] in order to compensate for the instability caused by the toxic sequences.
T135 929-1091 Sentence denotes The in vitro and in vivo properties of iPEDVPT-P5 were confirmed similarly to those of the parental PEDVPT-P5 strain in a seven-day-old conventional piglet model.
T136 1092-1217 Sentence denotes At 24–48 h post-electroporation, typical PEDV cytopathic effects of giant syncytia were observed for all recombinant viruses.
T137 1218-1438 Sentence denotes Viral stocks were prepared by passaging viruses in Vero cells one additional time (P1), and the presence of PEDV was confirmed by detection of PEDV S protein by immunofluorescence assay (Figure 1A) and sequence analyses.

2_test

Id Subject Object Predicate Lexical cue
31905842-28490591-144353228 851-853 28490591 denotes 23