PMC:7019868 / 17459-18897
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 315 | 92-96 | Species | denotes | PEDV | Tax:28295 |
| 316 | 232-236 | Species | denotes | PEDV | Tax:28295 |
| 317 | 150-157 | Chemical | denotes | iPEDVPT | |
| 318 | 428-435 | Chemical | denotes | iPEDVPT | |
| 319 | 485-492 | Chemical | denotes | iPEDVPT | |
| 320 | 724-731 | Chemical | denotes | iPEDVPT | |
| 321 | 732-734 | Chemical | denotes | P5 | MESH:C016883 |
| 322 | 739-746 | Chemical | denotes | iPEDVPT | |
| 323 | 968-978 | Chemical | denotes | iPEDVPT-P5 | |
| 328 | 1133-1137 | Species | denotes | PEDV | Tax:28295 |
| 329 | 1224-1230 | Species | denotes | stocks | Tax:3724 |
| 330 | 1326-1330 | Species | denotes | PEDV | Tax:28295 |
| 331 | 1160-1174 | Disease | denotes | giant syncytia | MESH:D005870 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T87 | 300-304 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
| T88 | 545-549 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
| T89 | 798-808 | Body_part | denotes | nucleotide | http://purl.org/sig/ont/fma/fma82740 |
| T90 | 1274-1279 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T91 | 1368-1375 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T176 | 62-63 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T177 | 129-130 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T178 | 143-148 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T179 | 245-247 | http://purl.obolibrary.org/obo/CLO_0001407 | denotes | 52 |
| T180 | 300-304 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | gene |
| T181 | 417-419 | http://purl.obolibrary.org/obo/CLO_0001407 | denotes | 52 |
| T182 | 457-464 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T183 | 545-549 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | gene |
| T184 | 571-573 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T185 | 631-638 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T186 | 776-777 | http://purl.obolibrary.org/obo/CLO_0001021 | denotes | B |
| T187 | 1049-1050 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T188 | 1098-1100 | http://purl.obolibrary.org/obo/CLO_0001382 | denotes | 48 |
| T189 | 1209-1216 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T190 | 1258-1265 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | viruses |
| T191 | 1269-1273 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T192 | 1269-1273 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T193 | 1274-1279 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T194 | 1301-1303 | http://purl.obolibrary.org/obo/CLO_0008285 | denotes | P1 |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T102 | 798-808 | Chemical | denotes | nucleotide | http://purl.obolibrary.org/obo/CHEBI_36976 |
| T103 | 1301-1303 | Chemical | denotes | P1 | http://purl.obolibrary.org/obo/CHEBI_60949 |
| T104 | 1368-1375 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T129 | 0-4 | Sentence | denotes | 3.1. |
| T130 | 5-34 | Sentence | denotes | Recovery of recombinant PEDVs |
| T131 | 35-272 | Sentence | denotes | We have recently developed a reverse genetic platform of PEDV and have successfully generated a recombinant virus, iPEDVPT-P96, which is phenotypically comparable to its parental attenuated Taiwan PEDV-Pintung 52 strain, PEDVPT-P96 [19]. |
| T132 | 273-575 | Sentence | denotes | To study the role of the S gene in the attenuation mechanism of PEDVPT-P96, we further generated the recombinant, highly virulent Taiwan PEDVPT 52 strain, iPEDVPT-P5, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) by replacing the complete sequence of the S gene reciprocally (Figure S1). |
| T133 | 576-692 | Sentence | denotes | In general, the approach to rescue the new recombinant viruses were virtually the same as published previously [19]. |
| T134 | 693-928 | Sentence | denotes | However, for the generation of iPEDVPT-P5 and iPEDVPT-P5-96S, we split the plasmid B to two fragments at nucleotide position of 9654 according to Hou et al. [23] in order to compensate for the instability caused by the toxic sequences. |
| T135 | 929-1091 | Sentence | denotes | The in vitro and in vivo properties of iPEDVPT-P5 were confirmed similarly to those of the parental PEDVPT-P5 strain in a seven-day-old conventional piglet model. |
| T136 | 1092-1217 | Sentence | denotes | At 24–48 h post-electroporation, typical PEDV cytopathic effects of giant syncytia were observed for all recombinant viruses. |
| T137 | 1218-1438 | Sentence | denotes | Viral stocks were prepared by passaging viruses in Vero cells one additional time (P1), and the presence of PEDV was confirmed by detection of PEDV S protein by immunofluorescence assay (Figure 1A) and sequence analyses. |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 31905842-28490591-144353228 | 851-853 | 28490591 | denotes | 23 |