PMC:7019868 / 15071-17051
Annnotations
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
264 | 128-131 | Species | denotes | pig | Tax:9823 |
265 | 396-404 | Chemical | denotes | formalin | MESH:D005557 |
266 | 412-420 | Chemical | denotes | paraffin | MESH:D010232 |
267 | 477-488 | Chemical | denotes | hematoxylin | MESH:D006416 |
268 | 493-498 | Chemical | denotes | eosin | MESH:D004801 |
269 | 500-503 | Chemical | denotes | H&E | |
288 | 691-695 | Species | denotes | PEDV | Tax:28295 |
289 | 1138-1142 | Species | denotes | goat | Tax:9925 |
290 | 1302-1306 | Species | denotes | PEDV | Tax:28295 |
291 | 1363-1367 | Species | denotes | goat | Tax:9925 |
292 | 1520-1526 | Species | denotes | rabbit | Tax:9986 |
293 | 1527-1532 | Species | denotes | mouse | Tax:10090 |
294 | 714-722 | Chemical | denotes | formalin | MESH:D005557 |
295 | 729-737 | Chemical | denotes | paraffin | MESH:D010232 |
296 | 795-801 | Chemical | denotes | xylene | MESH:D014992 |
297 | 834-841 | Chemical | denotes | ethanol | MESH:D000431 |
298 | 989-1009 | Chemical | denotes | Tris-buffered saline | |
299 | 1020-1028 | Chemical | denotes | Tween 20 | MESH:D011136 |
300 | 1030-1034 | Chemical | denotes | TBST | |
301 | 1448-1452 | Chemical | denotes | TBST | |
302 | 1558-1561 | Chemical | denotes | DAB | |
303 | 1668-1690 | Chemical | denotes | 3, 3′-diaminobenzidine | MESH:D015100 |
304 | 1692-1695 | Chemical | denotes | DAB | |
305 | 1789-1800 | Chemical | denotes | hematoxylin | MESH:D006416 |
LitCovid-PD-FMA-UBERON
Id | Subject | Object | Predicate | Lexical cue | fma_id |
---|---|---|---|---|---|
T73 | 306-314 | Body_part | denotes | Duodenum | http://purl.org/sig/ont/fma/fma7206 |
T74 | 316-323 | Body_part | denotes | jejunum | http://purl.org/sig/ont/fma/fma7207 |
T75 | 325-330 | Body_part | denotes | ileum | http://purl.org/sig/ont/fma/fma7208 |
T76 | 332-337 | Body_part | denotes | cecum | http://purl.org/sig/ont/fma/fma14541 |
T77 | 339-344 | Body_part | denotes | colon | http://purl.org/sig/ont/fma/fma14543 |
T78 | 346-352 | Body_part | denotes | rectum | http://purl.org/sig/ont/fma/fma14544 |
T79 | 357-379 | Body_part | denotes | mesenteric lymph nodes | http://purl.org/sig/ont/fma/fma277405 |
T80 | 747-754 | Body_part | denotes | tissues | http://purl.org/sig/ont/fma/fma9637 |
T81 | 921-925 | Body_part | denotes | Cell | http://purl.org/sig/ont/fma/fma68646 |
T82 | 1037-1043 | Body_part | denotes | tissue | http://purl.org/sig/ont/fma/fma9637 |
T83 | 1143-1148 | Body_part | denotes | serum | http://purl.org/sig/ont/fma/fma63083 |
T84 | 1309-1317 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T85 | 1368-1373 | Body_part | denotes | serum | http://purl.org/sig/ont/fma/fma63083 |
T86 | 1533-1547 | Body_part | denotes | immunoglobulin | http://purl.org/sig/ont/fma/fma62871 |
LitCovid-PD-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T8 | 306-314 | Body_part | denotes | Duodenum | http://purl.obolibrary.org/obo/UBERON_0002114 |
T9 | 316-323 | Body_part | denotes | jejunum | http://purl.obolibrary.org/obo/UBERON_0002115 |
T10 | 325-330 | Body_part | denotes | ileum | http://purl.obolibrary.org/obo/UBERON_0002116 |
T11 | 339-344 | Body_part | denotes | colon | http://purl.obolibrary.org/obo/UBERON_0001155 |
T12 | 346-352 | Body_part | denotes | rectum | http://purl.obolibrary.org/obo/UBERON_0001052 |
T13 | 357-379 | Body_part | denotes | mesenteric lymph nodes | http://purl.obolibrary.org/obo/UBERON_0002509 |
T14 | 368-373 | Body_part | denotes | lymph | http://purl.obolibrary.org/obo/UBERON_0002391 |
T15 | 1037-1043 | Body_part | denotes | tissue | http://purl.obolibrary.org/obo/UBERON_0000479 |
T16 | 1143-1148 | Body_part | denotes | serum | http://purl.obolibrary.org/obo/UBERON_0001977 |
T17 | 1368-1373 | Body_part | denotes | serum | http://purl.obolibrary.org/obo/UBERON_0001977 |
LitCovid-PD-CLO
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T157 | 100-105 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
T158 | 153-161 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | humanely |
T159 | 301-303 | http://purl.obolibrary.org/obo/CLO_0050507 | denotes | 22 |
T160 | 316-323 | http://purl.obolibrary.org/obo/UBERON_0002115 | denotes | jejunum |
T161 | 325-330 | http://purl.obolibrary.org/obo/UBERON_0002116 | denotes | ileum |
T162 | 339-344 | http://purl.obolibrary.org/obo/UBERON_0001155 | denotes | colon |
T163 | 346-352 | http://purl.obolibrary.org/obo/UBERON_0001052 | denotes | rectum |
T164 | 346-352 | http://purl.obolibrary.org/obo/UBERON_0006866 | denotes | rectum |
T165 | 357-379 | http://purl.obolibrary.org/obo/UBERON_0002509 | denotes | mesenteric lymph nodes |
T166 | 921-925 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cell |
T167 | 1138-1142 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T168 | 1213-1221 | http://purl.obolibrary.org/obo/CLO_0001658 | denotes | activity |
T169 | 1239-1246 | http://purl.obolibrary.org/obo/SO_0000418 | denotes | signals |
T170 | 1322-1327 | http://purl.obolibrary.org/obo/CLO_0001757 | denotes | 1, at |
T171 | 1328-1329 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T172 | 1363-1367 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T173 | 1527-1532 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
T174 | 1899-1906 | http://purl.obolibrary.org/obo/SO_0000418 | denotes | signals |
LitCovid-PD-CHEBI
Id | Subject | Object | Predicate | Lexical cue | chebi_id |
---|---|---|---|---|---|
T84 | 114-119 | Chemical | denotes | group | http://purl.obolibrary.org/obo/CHEBI_24433 |
T85 | 142-147 | Chemical | denotes | group | http://purl.obolibrary.org/obo/CHEBI_24433 |
T86 | 477-488 | Chemical | denotes | hematoxylin | http://purl.obolibrary.org/obo/CHEBI_51686 |
T87 | 696-703 | Chemical | denotes | antigen | http://purl.obolibrary.org/obo/CHEBI_59132 |
T88 | 795-801 | Chemical | denotes | xylene | http://purl.obolibrary.org/obo/CHEBI_27338 |
T89 | 834-841 | Chemical | denotes | ethanol | http://purl.obolibrary.org/obo/CHEBI_16236 |
T90 | 860-867 | Chemical | denotes | epitope | http://purl.obolibrary.org/obo/CHEBI_53000 |
T91 | 895-902 | Chemical | denotes | antigen | http://purl.obolibrary.org/obo/CHEBI_59132 |
T92 | 989-993 | Chemical | denotes | Tris | http://purl.obolibrary.org/obo/CHEBI_9754 |
T93 | 1020-1028 | Chemical | denotes | Tween 20 | http://purl.obolibrary.org/obo/CHEBI_53424 |
T94 | 1072-1080 | Chemical | denotes | hydrogen | http://purl.obolibrary.org/obo/CHEBI_49637 |
T95 | 1266-1273 | Chemical | denotes | antigen | http://purl.obolibrary.org/obo/CHEBI_59132 |
T96 | 1319-1321 | Chemical | denotes | DE | http://purl.obolibrary.org/obo/CHEBI_73828 |
T97 | 1558-1561 | Chemical | denotes | DAB | http://purl.obolibrary.org/obo/CHEBI_90994 |
T98 | 1692-1695 | Chemical | denotes | DAB | http://purl.obolibrary.org/obo/CHEBI_90994 |
T99 | 1697-1706 | Chemical | denotes | chromogen | http://purl.obolibrary.org/obo/CHEBI_75050 |
T100 | 1789-1800 | Chemical | denotes | hematoxylin | http://purl.obolibrary.org/obo/CHEBI_51686 |
LitCovid-PD-GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T38 | 1202-1221 | http://purl.obolibrary.org/obo/GO_0004601 | denotes | peroxidase activity |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T111 | 0-6 | Sentence | denotes | 2.5.2. |
T112 | 7-46 | Sentence | denotes | Histopathology and Immunohistochemistry |
T113 | 47-305 | Sentence | denotes | At three days post-inoculation, three pigs from each virus-treated group and one pig from mock group were humanely euthanized by electrocution followed by exsanguination for histopathological and immunohistochemical assessments, as described previously [22]. |
T114 | 306-623 | Sentence | denotes | Duodenum, jejunum, ileum, cecum, colon, rectum and mesenteric lymph nodes were collected, formalin-fixed, paraffin-embedded, sectioned at 4 μm, and stained routinely with hematoxylin and eosin (H&E) for morphometric analysis by assessing the ratio of villi height to crypt depth blindly by one veterinary pathologist. |
T115 | 624-704 | Sentence | denotes | Immunohistochemistry was performed to evaluate the distribution of PEDV antigen. |
T116 | 705-952 | Sentence | denotes | Briefly, formalin-fixed paraffin-embedded tissues were sectioned at 4 μm, deparaffined in xylene, rehydrated in serially diluted ethanol, and proceeded to epitope retrieval with the Trilogy antigen retrieval system (Cell Marque, Rocklin, CA, USA). |
T117 | 953-1261 | Sentence | denotes | After being washed three times with Tris-buffered saline plus 0.1% Tween 20 (TBST), tissue slides were treated with 3% hydrogen peroxidase (KYB, New Taipei City, Taiwan) and 10% normal goat serum (Dako, Carpinteria, CA, USA) to block the endogenous peroxidase activity and non-specific signals, respectively. |
T118 | 1262-1453 | Sentence | denotes | For antigen detection, an in-house anti-PEDV N antibody, DE-1, at a dilution of 1:1000 in 10% normal goat serum was applied to the slides for 1 h at RT followed by three times wash with TBST. |
T119 | 1454-1752 | Sentence | denotes | The first antibodies were then captured using the polyclonal anti-rabbit/mouse immunoglobulin, EnVision-DAB+ system (Agilent Technologies, Santa Clara, CA, USA) at RT for 1 h and color was developed afterward with 3, 3′-diaminobenzidine (DAB) chromogen (Agilent Technologies, Santa Clara, CA, USA). |
T120 | 1753-1889 | Sentence | denotes | The slides were counterstained with hematoxylin (MUTO, Tokyo, Japan), mounted in Entellan (Merck, Darmstadt, Germany) and cover slipped. |
T121 | 1890-1980 | Sentence | denotes | Positive signals were visualized under an inverted light microscope (Nikon, Tokyo, Japan). |
2_test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
31905842-30089548-144353227 | 301-303 | 30089548 | denotes | 22 |