PMC:7019868 / 12181-12954
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 220 | 228-232 | Chemical | denotes | DPBS | |
| 221 | 304-311 | Chemical | denotes | agarose | MESH:D012685 |
| 222 | 476-484 | Chemical | denotes | formalin | MESH:D005557 |
| 223 | 565-579 | Chemical | denotes | crystal violet | MESH:D005840 |
| 224 | 587-594 | Chemical | denotes | ethanol | MESH:D000431 |
| 225 | 609-614 | Chemical | denotes | water | MESH:D014867 |
| 226 | 677-691 | Chemical | denotes | crystal violet | MESH:D005840 |
| 227 | 726-731 | Chemical | denotes | water | MESH:D014867 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T64 | 180-185 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T65 | 428-433 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T143 | 175-179 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T144 | 175-179 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T145 | 180-185 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T146 | 428-433 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T65 | 280-282 | Chemical | denotes | PI | http://purl.obolibrary.org/obo/CHEBI_28874|http://purl.obolibrary.org/obo/CHEBI_53806|http://purl.obolibrary.org/obo/CHEBI_61484|http://purl.obolibrary.org/obo/CHEBI_74790 |
| T69 | 304-311 | Chemical | denotes | agarose | http://purl.obolibrary.org/obo/CHEBI_2511 |
| T70 | 565-579 | Chemical | denotes | crystal violet | http://purl.obolibrary.org/obo/CHEBI_41688 |
| T71 | 587-594 | Chemical | denotes | ethanol | http://purl.obolibrary.org/obo/CHEBI_16236 |
| T72 | 609-614 | Chemical | denotes | water | http://purl.obolibrary.org/obo/CHEBI_15377 |
| T73 | 677-691 | Chemical | denotes | crystal violet | http://purl.obolibrary.org/obo/CHEBI_41688 |
| T74 | 692-700 | Chemical | denotes | solution | http://purl.obolibrary.org/obo/CHEBI_75958 |
| T75 | 726-731 | Chemical | denotes | water | http://purl.obolibrary.org/obo/CHEBI_15377 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T87 | 0-94 | Sentence | denotes | Plaque assays were performed as previously described [19] to characterize plaque morphologies. |
| T88 | 95-312 | Sentence | denotes | Briefly, after absorption of PEDVs at an MOI of 0.0001, confluent monolayers of Vero cells in six-well plates were washed twice with DPBS and then covered with an overlay of pre-warmed PI medium containing 1% agarose. |
| T89 | 313-423 | Sentence | denotes | After solidification of the overlays, the plates were incubated at 37 °C for 72 h to produce distinct plaques. |
| T90 | 424-532 | Sentence | denotes | The cells were then fixed in 3.16% neutral-buffered formalin for 1 h before removing the semisolid overlays. |
| T91 | 533-625 | Sentence | denotes | The plates were stained with 1% crystal violet in 20% ethanol and distilled water for 1 min. |
| T92 | 626-773 | Sentence | denotes | Viral plaques were inspected after washing off the crystal violet solution, rinsing the plates with water, and air-drying at room temperature (RT). |