PMC:7019868 / 11249-12954
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 207 | 270-306 | Chemical | denotes | Dulbecco’s phosphate-buffered saline | |
| 208 | 308-312 | Chemical | denotes | DPBS | |
| 209 | 332-341 | Chemical | denotes | PI medium | |
| 210 | 603-607 | Chemical | denotes | DPBS | |
| 211 | 150-158 | Disease | denotes | infected | MESH:D007239 |
| 220 | 1160-1164 | Chemical | denotes | DPBS | |
| 221 | 1236-1243 | Chemical | denotes | agarose | MESH:D012685 |
| 222 | 1408-1416 | Chemical | denotes | formalin | MESH:D005557 |
| 223 | 1497-1511 | Chemical | denotes | crystal violet | MESH:D005840 |
| 224 | 1519-1526 | Chemical | denotes | ethanol | MESH:D000431 |
| 225 | 1541-1546 | Chemical | denotes | water | MESH:D014867 |
| 226 | 1609-1623 | Chemical | denotes | crystal violet | MESH:D005840 |
| 227 | 1658-1663 | Chemical | denotes | water | MESH:D014867 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T58 | 86-91 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T59 | 134-139 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T60 | 236-241 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T61 | 447-452 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T62 | 494-500 | Body_part | denotes | tissue | http://purl.org/sig/ont/fma/fma9637 |
| T63 | 556-561 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T64 | 1112-1117 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T65 | 1360-1365 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T4 | 494-500 | Body_part | denotes | tissue | http://purl.obolibrary.org/obo/UBERON_0000479 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T17 | 509-519 | Disease | denotes | infectious | http://purl.obolibrary.org/obo/MONDO_0005550 |
| T18 | 887-896 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T129 | 81-85 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T130 | 81-85 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T131 | 86-91 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T132 | 134-139 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T133 | 169-174 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T134 | 178-179 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T135 | 236-241 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T136 | 442-446 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T137 | 442-446 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T138 | 447-452 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T139 | 551-555 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T140 | 551-555 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T141 | 556-561 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T142 | 632-636 | http://purl.obolibrary.org/obo/CLO_0001550 | denotes | a 10 |
| T143 | 1107-1111 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T144 | 1107-1111 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T145 | 1112-1117 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T146 | 1360-1365 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T53 | 281-290 | Chemical | denotes | phosphate | http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474 |
| T57 | 332-334 | Chemical | denotes | PI | http://purl.obolibrary.org/obo/CHEBI_28874|http://purl.obolibrary.org/obo/CHEBI_53806|http://purl.obolibrary.org/obo/CHEBI_61484|http://purl.obolibrary.org/obo/CHEBI_74790 |
| T61 | 812-814 | Chemical | denotes | PI | http://purl.obolibrary.org/obo/CHEBI_28874|http://purl.obolibrary.org/obo/CHEBI_53806|http://purl.obolibrary.org/obo/CHEBI_61484|http://purl.obolibrary.org/obo/CHEBI_74790 |
| T65 | 1212-1214 | Chemical | denotes | PI | http://purl.obolibrary.org/obo/CHEBI_28874|http://purl.obolibrary.org/obo/CHEBI_53806|http://purl.obolibrary.org/obo/CHEBI_61484|http://purl.obolibrary.org/obo/CHEBI_74790 |
| T69 | 1236-1243 | Chemical | denotes | agarose | http://purl.obolibrary.org/obo/CHEBI_2511 |
| T70 | 1497-1511 | Chemical | denotes | crystal violet | http://purl.obolibrary.org/obo/CHEBI_41688 |
| T71 | 1519-1526 | Chemical | denotes | ethanol | http://purl.obolibrary.org/obo/CHEBI_16236 |
| T72 | 1541-1546 | Chemical | denotes | water | http://purl.obolibrary.org/obo/CHEBI_15377 |
| T73 | 1609-1623 | Chemical | denotes | crystal violet | http://purl.obolibrary.org/obo/CHEBI_41688 |
| T74 | 1624-1632 | Chemical | denotes | solution | http://purl.obolibrary.org/obo/CHEBI_75958 |
| T75 | 1658-1663 | Chemical | denotes | water | http://purl.obolibrary.org/obo/CHEBI_15377 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T29 | 7-13 | http://purl.obolibrary.org/obo/GO_0040007 | denotes | Growth |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T79 | 0-6 | Sentence | denotes | 2.4.3. |
| T80 | 7-56 | Sentence | denotes | Growth Kinetics, Viral Titration and Plaque Assay |
| T81 | 57-231 | Sentence | denotes | Confluent monolayers of Vero cells were seeded onto six-well plates (5 × 105 cells/well) and infected with each virus at a MOI of 1 and 0.001 at 37 °C for 1 h in triplicates. |
| T82 | 232-342 | Sentence | denotes | The cells were then washed twice with Dulbecco’s phosphate-buffered saline (DPBS) and maintained in PI medium. |
| T83 | 343-540 | Sentence | denotes | The supernatants at indicated time points were collected and proceeded for viral quantification on Vero cells in 96-well plates using the standard 50% tissue-culture infectious dose (TCID50) assay. |
| T84 | 541-744 | Sentence | denotes | In brief, Vero cells in 96-well plates were washed twice with DPBS and then incubated with a 10-fold serially diluted culture supernatant acquired from the aforementioned six-well plate at 37 °C for 1 h. |
| T85 | 745-846 | Sentence | denotes | After absorption, the inoculum was removed and replaced with fresh PI-medium following one wash step. |
| T86 | 847-931 | Sentence | denotes | The titers were determined at 72 h post-infection using the Reed–Muench method [21]. |
| T87 | 932-1026 | Sentence | denotes | Plaque assays were performed as previously described [19] to characterize plaque morphologies. |
| T88 | 1027-1244 | Sentence | denotes | Briefly, after absorption of PEDVs at an MOI of 0.0001, confluent monolayers of Vero cells in six-well plates were washed twice with DPBS and then covered with an overlay of pre-warmed PI medium containing 1% agarose. |
| T89 | 1245-1355 | Sentence | denotes | After solidification of the overlays, the plates were incubated at 37 °C for 72 h to produce distinct plaques. |
| T90 | 1356-1464 | Sentence | denotes | The cells were then fixed in 3.16% neutral-buffered formalin for 1 h before removing the semisolid overlays. |
| T91 | 1465-1557 | Sentence | denotes | The plates were stained with 1% crystal violet in 20% ethanol and distilled water for 1 min. |
| T92 | 1558-1705 | Sentence | denotes | Viral plaques were inspected after washing off the crystal violet solution, rinsing the plates with water, and air-drying at room temperature (RT). |