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PMC:6988269 / 5607-6533 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T14 35-38 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T15 430-435 Body_part denotes serum http://purl.org/sig/ont/fma/fma63083

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T4 430-435 Body_part denotes serum http://purl.obolibrary.org/obo/UBERON_0001977

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T49 0-1 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T50 347-348 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T51 606-612 http://purl.obolibrary.org/obo/CLO_0001929 denotes Berlin
T52 738-740 http://purl.obolibrary.org/obo/CLO_0053799 denotes 45
T53 875-886 http://purl.obolibrary.org/obo/OBI_0000968 denotes instruments

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T7 64-70 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T8 260-278 Chemical denotes magnesium sulphate http://purl.obolibrary.org/obo/CHEBI_32599
T9 260-269 Chemical denotes magnesium http://purl.obolibrary.org/obo/CHEBI_25107
T10 270-278 Chemical denotes sulphate http://purl.obolibrary.org/obo/CHEBI_16189
T11 315-322 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T12 355-373 Chemical denotes magnesium sulphate http://purl.obolibrary.org/obo/CHEBI_32599
T13 355-364 Chemical denotes magnesium http://purl.obolibrary.org/obo/CHEBI_25107
T14 365-373 Chemical denotes sulphate http://purl.obolibrary.org/obo/CHEBI_16189
T15 374-382 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T16 464-469 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406
T17 543-559 Chemical denotes oligonucleotides http://purl.obolibrary.org/obo/CHEBI_7754
T18 828-833 Chemical denotes Light http://purl.obolibrary.org/obo/CHEBI_30212

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T6 297-310 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T7 297-310 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T8 678-699 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse transcription
T9 686-699 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T42 0-452 Sentence denotes A 25 μL reaction contained 5 μL of RNA, 12.5 μL of 2 × reaction buffer provided with the Superscript III one step RT-PCR system with Platinum Taq Polymerase (Invitrogen, Darmstadt, Germany; containing 0.4 mM of each deoxyribont triphosphates (dNTP) and 3.2 mM magnesium sulphate), 1 μL of reverse transcriptase/Taq mixture from the kit, 0.4 μL of a 50 mM magnesium sulphate solution (Invitrogen), and 1 μg of nonacetylated bovine serum albumin (Roche).
T43 453-538 Sentence denotes Primer and probe sequences, as well as optimised concentrations are shown in Table 1.
T44 539-623 Sentence denotes All oligonucleotides were synthesised and provided by Tib-Molbiol (Berlin, Germany).
T45 624-782 Sentence denotes Thermal cycling was performed at 55 °C for 10 min for reverse transcription, followed by 95 °C for 3 min and then 45 cycles of 95 °C for 15 s, 58 °C for 30 s.
T46 783-926 Sentence denotes Participating laboratories used either Roche Light Cycler 480II or Applied Biosystems ViiA7 instruments (Applied Biosystems, Hong Kong, China).

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
115 430-443 Gene denotes serum albumin Gene:213
116 332-335 Gene denotes kit Gene:3815
117 543-559 Chemical denotes oligonucleotides MESH:D009841