PMC:6891841 / 1970-3199 JSONTXT

{"project":"testtesttest","denotations":[{"id":"T33","span":{"begin":174,"end":193},"obj":"Body_part"},{"id":"T34","span":{"begin":340,"end":353},"obj":"Body_part"},{"id":"T35","span":{"begin":473,"end":492},"obj":"Body_part"},{"id":"T36","span":{"begin":925,"end":929},"obj":"Body_part"},{"id":"T37","span":{"begin":1051,"end":1055},"obj":"Body_part"},{"id":"T38","span":{"begin":1199,"end":1210},"obj":"Body_part"},{"id":"T39","span":{"begin":1216,"end":1228},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"uberon_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CL_0002608"},{"id":"A34","pred":"uberon_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/GO_0005622"},{"id":"A35","pred":"uberon_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/CL_0002608"},{"id":"A36","pred":"uberon_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/UBERON_0000033"},{"id":"A37","pred":"uberon_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A38","pred":"uberon_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/UBERON_0002049"},{"id":"A39","pred":"uberon_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/CL_0000738"}],"text":"At the core of the issue is selective vulnerability, that is, why some neurons are vulnerable relative to others. Experiments began to focus on characteristics of individual hippocampal neurons to understand this issue, made possible by the increasing acceptance of the hippocampal slice preparation and with it, the ability to make stable intracellular recordings. Using improved markers of single cells such as biocytin, the basic neuronal properties and morphologies of hippocampal neurons were clarified, and an increasingly detailed map of their circuitry emerged. This understanding proceeded in parallel with cellular investigations of induced seizure-like activity in slices of normal animals, leading to predictions about how the characteristics of the cells and circuitry contributed to seizures. The techniques have now been superseded by more advanced imaging and recording methods, including recordings in awake head-fixed animals, as well as viral-based strategies and techniques to record simultaneously from many areas rather than one cell. Therefore, the understanding of cells and their circuitry continues, but the “neurocentric” view has given way to one that includes glia, the vasculature, and immune cells."}