PMC:6879884 / 18410-20027 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6879884","sourcedb":"PMC","sourceid":"6879884","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6879884","text":"Fig. 4. Förster resonance energy transfer (FRET) ratio [enhanced cyan fluorescent protein (eCFP)/citrine] was measured over a period of 6 min using the glucose FRET sensor FLII12Pglu-700µΔ6. A: H441 cells grown on coverslips were exposed to either osmotically balanced 5 mM glucose (●) or 15 mM glucose (▲), both n = 16 experiments. Cells were also exposed to the same conditions in the presence of the hexokinase inhibitor 3-bromopyruvic acid (BrPy; ○ or △, respectively), both n = 14 experiments. B: FRET ratio (eCFP/Citrine) for H441 cells grown at air-liquid interface and exposed to either 5 mM glucose (●, n = 12 experiments) or 15 mM glucose (▲, n = 6 experiments). Cells were also exposed to 5 mM glucose in the presence of the hexokinase inhibitor BrPy (○, n = 4 experiments). C: FRET ratio (eCFP/Citrine) in human bronchial epithelial cells (HBECs) grown at air-liquid interface, exposed to either osmotically balanced 5 mM glucose (●, n = 12 experiments) or 15 mM glucose (▲, n = 15 experiments). D: FRET ratio (eCFP/Citrine)-glucose dose-response curve for cells shown in A, equilibrated with extracellular glucose as described in results. Data points are shown as means only in A, B, and C for clarity. Data in D are shown as means ± SD. Data were fitted with a sigmoidal one-site binding curve (df = 37, r2 = 0.6). Values shown in A and B are directly comparable, but FRET ratio values in A, B, and C cannot be directly compared because of the different imaging conditions required for the two cell types and their growth substrates. ****Significantly different between groups as indicated, P \u003c 0.0001.","divisions":[{"label":"label","span":{"begin":0,"end":7}}],"tracks":[]}