PMC:6879884 / 15522-16255 JSONTXT

{"project":"2_test","denotations":[{"id":"31433692-27897253-2122381","span":{"begin":257,"end":259},"obj":"27897253"}],"text":"Using the Seahorse assay, we previously showed that airway cells produce energy by mitochondrial respiration (OCR) and that elevation of extracellular glucose shifts metabolism to glycolysis (ECAR), which is associated with increased lactic acid secretion (12). We found that BrPy (100 μM) was effective at inhibiting both mitochondrial respiration (Fig. 3A) and glycolysis in these cells (Fig. 3, B and C). We calculated that BrPy inhibited glycolysis with an IC50 of 0.06 ± 0.02 mM (Fig. 3D). Application of 2-DG, an inhibitor of all hexokinase activity, was more effective at inhibiting respiration and glycolysis (Fig. 3, A–C). These data indicate that glycolysis is predominantly driven by hexokinase II activity in these cells."}