PMC:6610382 / 3125-4475 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6610382","sourcedb":"PMC","sourceid":"6610382","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6610382","text":"Although many groups have attempted this approach for different disorders, Srivastava et al have made an exceptional amount of progress in applying this method to the problem of epilepsy. To begin, they performed RNA sequencing on the hippocampi of 100 mice treated with pilocarpine (a model for temporal lobe epilepsy [TLE]) and 100 control animals. They then used computer algorithms in order to find groups of genes that changed in the same direction in the different animals. This approach generated “modules” that behaved similarly (so that if a given mouse expressed 50% more of gene “X,” the other genes in the module were also increased in their expression). Overall, they found 28 modules representing clusters of genes related to distinct functions such as “inflammation” and “synaptic transmission.” Afterward, they correlated the change in each module’s expression with the seizure frequency of the mice (prior to their sacrifice for RNA sequencing) and found that increasing activity in the inflammation module was positively correlated with seizure frequency. To validate their approach, they performed a similar analysis on human neurosurgical specimens. They observed that the same pattern of gene modules emerged in the human samples, suggesting that the research team had indeed identified genetic signatures of the epileptic brain.","tracks":[{"project":"testtesttest","denotations":[{"id":"T13","span":{"begin":296,"end":309},"obj":"Body_part"},{"id":"T14","span":{"begin":1344,"end":1349},"obj":"Body_part"}],"attributes":[{"id":"A13","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/UBERON_0001871"},{"id":"A14","pred":"uberon_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A15","pred":"uberon_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"subj":"T13","pred":"source","obj":"testtesttest"},{"subj":"T14","pred":"source","obj":"testtesttest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"testtesttest","color":"#ecd793","default":true}]}]}}