PMC:6599329 / 30915-31279 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6599329","sourcedb":"PMC","sourceid":"6599329","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6599329","text":"One hundred milliliter of diluted culture was dispensed each in 250 ml conical flasks for two different time points (12 h, 24 h) in triplicates and incubated at 37 °C with shaking at 150 rpm. After proper incubation, the cells were pelleted and washed twice with sterile ice-cold phosphate buffered saline (PBS, pH 7.4) and stored at -80 °C till protein isolation.","tracks":[]}