PMC:6599329 / 16666-17836 JSONTXT

{"project":"2_test","denotations":[{"id":"31253082-29358339-12221358","span":{"begin":516,"end":517},"obj":"29358339"},{"id":"31253082-15947432-12221359","span":{"begin":956,"end":958},"obj":"15947432"},{"id":"31253082-28285095-12221359","span":{"begin":956,"end":958},"obj":"28285095"}],"text":"In the present investigation, various enzymes including chorismate synthase (3.97 fold), 3-phosphoshikimate 1-carboxyvinyltransferase (1.5 fold), and 3-deoxy-7-phosphoheptulonate synthase (1.56 fold) which are involved in the biosynthesis of aromatic amino acids were found to be upregulated in biofilm stages. A similar trend of enhanced expression of aromatic amino acid biosynthesis enzymes has already been reported in a previous proteomic study performed on strong and weak biofilm forming E. faecalis strains [8]. Other major amino acid biosynthesis enzymes upregulated were Succinyl-diaminopimelate desuccinylase (6.35 fold), MTA/SAH nucleosidase (2.88 fold), cysteine synthase A (3.387 fold), Glutamine synthetase (5.81 fold), Acetolactate synthase (1.89 fold), etc. Previous studies on several other bacterial genera also revealed the role of amino acids in the formation of tight microcolonies and in maintaining the stability of mature biofilm [10–12]. Hence it is evident that during the biofilm maturation process, a series of amino acids and its related metabolites will be upregulated for contributing to extracellular matrix component of robust biofilms."}