PMC:6599329 / 14084-14890 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6599329","sourcedb":"PMC","sourceid":"6599329","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6599329","text":"Validation of selected biofilm associated genes by RT-PCR\nThe genes rfbB (Rhamnose biosynthesis), arcA (Arginine deiminase), luxS (Quorum sensing), cAD1 (Pheromone cAD1 lipoprotein) and fbn (Fibrinogen/Fibronectin binding protein associated with adhesion) were selected to monitor the fold change expression in biofilm stage when compared to planktonic stage and the results are shown in Fig. 4. All these five genes were found to be upregulated and adhesion protein (fbn) showed a comparatively large fold increase in expression. This substantiates the significant role of these pathways in the development of mature biofilm.\nFig. 4 Real time qPCR validation of selected biofilm associated genes. Fold change expression of genes observed in biofilm stage with respect to its corresponding planktonic stage","divisions":[{"label":"title","span":{"begin":0,"end":57}},{"label":"label","span":{"begin":627,"end":633}}],"tracks":[]}