PMC:64779 / 20499-21638 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/64779","sourcedb":"PMC","sourceid":"64779","source_url":"https://www.ncbi.nlm.nih.gov/pmc/64779","text":"MID1 \u0026 MID2 can form heterodimers on microtubules\nGiven their high level of identity, we investigated whether MID1 and MID2 can also form heterodimers using the yeast two-hybrid system, immunofluorescence of transiently transfected Cos1 cells and co-immunoprecipitation. Co-transformation of the yeast MaV203 strain with both pDBLeu-MID1 and pPC86-MID2 (or in the reverse vector combination) resulted in high level activation of all reporter genes indicating a strong interaction that was comparable to the strength of the MID1-MID1 homo-interaction (Fig 3A). These findings are contradictory to initial reports from a study by Cainarca et al [17] but have been confirmed in experiments involving transient transfection of GFP-MID1 and myc-MID2 fusion constructs (Fig 3B) as well as by co-immunoprecipitation (Fig 3C). Introduction of individual domain-deletions of MID1 together with full-length or domain-deletion MID2, and vice versa, into Cos1 cells and the yeast strain MaV203 using the relevant constructs showed, as expected, that the coiled-coil motif was largely responsible for mediating this heterodimerisation (data not shown).","divisions":[{"label":"Title","span":{"begin":0,"end":49}}],"tracks":[{"project":"2_test","denotations":[{"id":"11806752-10400985-9188082","span":{"begin":644,"end":646},"obj":"10400985"}],"attributes":[{"subj":"11806752-10400985-9188082","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecc2","default":true}]}]}}