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{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6365940","sourcedb":"PMC","sourceid":"6365940","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6365940","text":"Fig. 5 Digoxin inhibited Nrf2 by decreasing Nrf2 mRNA at transcriptional level in SW1990/Gem and Panc-1/Gem cells. (A–D) Quantitative real-time PCR analysis. (E–F) SW1990/Gem and Panc-1/Gem cells were treated with 80 nM of digoxin, 20 µM of MG132, or a combination of digoxin and MG132 for 6 h, the protein level of Nrf2 were detected by Western blot. (G–H) CHX-chase analysis. (I–K) Ubiquitination assay. Data were expressed as mean ± SD, and the results were representative of three independent experiments. Significant differences were indicated as **P \u003c 0.01, ***P \u003c 0.001 vs. control group, ###P \u003c 0.001 vs. MG132 group. N.S., no significant.","divisions":[{"label":"label","span":{"begin":0,"end":6}}],"tracks":[]}