PMC:6365940 / 10747-12726 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6365940","sourcedb":"PMC","sourceid":"6365940","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6365940","text":"RNA samples were reverse transcribed to cDNA, and then quantitative PCR reaction was performed using AceQ qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) in the LightCycler1 96 Real-Time PCR System (Roche, Basel, Swiss). The primer sequences used in this study were shown in Table 1.\nTable 1 The sequences of primers used in the study.\nGene Forward (5′–3′) Reverse (5′–3′)\nNrf2 CAGCTTTTGGCGCAGACATT GACTGGGCTCTCGATGTGAC\nKeap1 ATCGATGGCCACATCTATG GATCCTTCGTGTCAGCATTG\nHO-1 CTTTCAGAAGGGCCAGGTGA GTAGACAGGGGCGAAGACTG\nNQO1 GGTTTGGAGTCCCTGCCATT TTGCAGAGAGTACATGGAGCC\nPRDX1 CCCACGGAGATCATTGTT CGAGATGCCTTCATCAGCCT\nPRDX2 GAAGCTGTCGGACTACAAAGG TCGGTGGGGCACACAAAAG\nPRDX3 GCCGTTGTCAATGGAGAGTT CAACAGCGTCAGAGTCACCT\nPRDX4 AGAGGAGTGCCACTTCTACG GGAAATCTTCGCTTTGCTTAGGT\nSRXN1 AGTTTTAGGGTACAGTTTGGCTAGGTATC AGTGGTACTTGTGCTAGGCAT ATTAGTAA\nSOD2 TGGGGTTGGCTTGGTTTCAA GGAATAAGGCCTGTTGTTCCTTG\nCAT CGGAGATTCAACACTGCCAATG TTCTTGACCGCTTTCTTCTGGA\nGCLC GGACAAGAATACACCATCTCCA ATACTGCAGGCTTGGAATGTC\nGCLM GGGAACCTGCTGAACTGG CTGGGTTGATTTGGGAACTC\nGSR AGGAGCTGGAGAACGCTGGC CAATGGCCCAGAGCAGGCA\nGSTA2 GGCTGCAGCTGGAGTAGAGT AAGGCAGGGAAGTAGCGATT\nGSTA4 GGCAGCAAGGCCCAAGCTCCACT GGCCTAAAGATGTTGTAGACGG\nGSTM2 ACA ACCTGTGCGGGGAATC AGCTTCAGCATTTCAGGGAGTG\nGSTM3 GACTTTCCTAATCTGCCCTACCTC TTCTTCTTCAGTCTCACCACACAT\nAKR1B1 TATTCACTGGCCGACTGGCTTTA GAACCACATTGCCCGACTCA\nAKR1B10 GCAGGACGTGAGACTTCTACC ATCCTGCATCAATGGCCACC\nAKR1C1 TAGCCTGTGAGGGAGGAAGAA TTGCCAATTTGGTGGCCTCT\nAKR1C3 GGATTTGGCACCTATGCACCTC CTATATGGCGGAACCCAGCTTCTA\nALDH1A1 ACTCCCAAGCACGCTTAGTGCTC TCGTCATGTCTTAGCCAGCT\nALDH3A1 ACTGGGCGTGGTCCTCGTCATTGG GTGAGGATGGTGGGGGCTATGTAG\nABCC1 CATTGGCGAGCCTGGTAG TCGTAGGAGTGTCCGTGGAT\nABCC2 CTTGGGCTTCCTATGGCTCC ATCGAACAGCAGGGACTGTG\nABCC3 CAGAGAAGGTGCAGGTGACA CTAAAGCAGCATAGACGCCC\nABCC5 GTTCAGGAGAACTCGACCGTTGG TTTGGAAGTAGTCCGGATGGGCTT\nIDH1 TGCAAAAATATCCCCCGGCT TACATCCCCATGGCAACACC\nME1 CTGCCTGTCATTCTGGATGT ACCTCTTACTCTTCTCTGCC\nPGD ATTCTCAAGTTCCAAGACACCG GTGGTAAAACAGGGCATGGGA\nGAPDH CTGACTTCAACAGCGACACC TGCTGTAGCCAAATTCGTTGT","divisions":[{"label":"label","span":{"begin":288,"end":295}},{"label":"caption","span":{"begin":296,"end":339}},{"label":"p","span":{"begin":296,"end":339}},{"label":"tr","span":{"begin":340,"end":376}},{"label":"th","span":{"begin":340,"end":344}},{"label":"th","span":{"begin":345,"end":360}},{"label":"th","span":{"begin":361,"end":376}},{"label":"tr","span":{"begin":377,"end":423}},{"label":"td","span":{"begin":377,"end":381}},{"label":"td","span":{"begin":382,"end":402}},{"label":"td","span":{"begin":403,"end":423}},{"label":"tr","span":{"begin":424,"end":470}},{"label":"td","span":{"begin":424,"end":429}},{"label":"td","span":{"begin":430,"end":449}},{"label":"td","span":{"begin":450,"end":470}},{"label":"tr","span":{"begin":471,"end":517}},{"label":"td","span":{"begin":471,"end":475}},{"label":"td","span":{"begin":476,"end":496}},{"label":"td","span":{"begin":497,"end":517}},{"label":"tr","span":{"begin":518,"end":565}},{"label":"td","span":{"begin":518,"end":522}},{"label":"td","span":{"begin":523,"end":543}},{"label":"td","span":{"begin":544,"end":565}},{"label":"tr","span":{"begin":566,"end":611}},{"label":"td","span":{"begin":566,"end":571}},{"label":"td","span":{"begin":572,"end":590}},{"label":"td","span":{"begin":591,"end":611}},{"label":"tr","span":{"begin":612,"end":659}},{"label":"td","span":{"begin":612,"end":617}},{"label":"td","span":{"begin":618,"end":639}},{"label":"td","span":{"begin":640,"end":659}},{"label":"tr","span":{"begin":660,"end":707}},{"label":"td","span":{"begin":660,"end":665}},{"label":"td","span":{"begin":666,"end":686}},{"label":"td","span":{"begin":687,"end":707}},{"label":"tr","span":{"begin":708,"end":758}},{"label":"td","span":{"begin":708,"end":713}},{"label":"td","span":{"begin":714,"end":734}},{"label":"td","span":{"begin":735,"end":758}},{"label":"tr","span":{"begin":759,"end":825}},{"label":"td","span":{"begin":759,"end":764}},{"label":"td","span":{"begin":765,"end":794}},{"label":"td","span":{"begin":795,"end":825}},{"label":"tr","span":{"begin":826,"end":875}},{"label":"td","span":{"begin":826,"end":830}},{"label":"td","span":{"begin":831,"end":851}},{"label":"td","span":{"begin":852,"end":875}},{"label":"tr","span":{"begin":876,"end":925}},{"label":"td","span":{"begin":876,"end":879}},{"label":"td","span":{"begin":880,"end":902}},{"label":"td","span":{"begin":903,"end":925}},{"label":"tr","span":{"begin":926,"end":975}},{"label":"td","span":{"begin":926,"end":930}},{"label":"td","span":{"begin":931,"end":953}},{"label":"td","span":{"begin":954,"end":975}},{"label":"tr","span":{"begin":976,"end":1020}},{"label":"td","span":{"begin":976,"end":980}},{"label":"td","span":{"begin":981,"end":999}},{"label":"td","span":{"begin":1000,"end":1020}},{"label":"tr","span":{"begin":1021,"end":1065}},{"label":"td","span":{"begin":1021,"end":1024}},{"label":"td","span":{"begin":1025,"end":1045}},{"label":"td","span":{"begin":1046,"end":1065}},{"label":"tr","span":{"begin":1066,"end":1113}},{"label":"td","span":{"begin":1066,"end":1071}},{"label":"td","span":{"begin":1072,"end":1092}},{"label":"td","span":{"begin":1093,"end":1113}},{"label":"tr","span":{"begin":1114,"end":1166}},{"label":"td","span":{"begin":1114,"end":1119}},{"label":"td","span":{"begin":1120,"end":1143}},{"label":"td","span":{"begin":1144,"end":1166}},{"label":"tr","span":{"begin":1167,"end":1216}},{"label":"td","span":{"begin":1167,"end":1172}},{"label":"td","span":{"begin":1173,"end":1193}},{"label":"td","span":{"begin":1194,"end":1216}},{"label":"tr","span":{"begin":1217,"end":1272}},{"label":"td","span":{"begin":1217,"end":1222}},{"label":"td","span":{"begin":1223,"end":1247}},{"label":"td","span":{"begin":1248,"end":1272}},{"label":"tr","span":{"begin":1273,"end":1324}},{"label":"td","span":{"begin":1273,"end":1279}},{"label":"td","span":{"begin":1280,"end":1303}},{"label":"td","span":{"begin":1304,"end":1324}},{"label":"tr","span":{"begin":1325,"end":1375}},{"label":"td","span":{"begin":1325,"end":1332}},{"label":"td","span":{"begin":1333,"end":1354}},{"label":"td","span":{"begin":1355,"end":1375}},{"label":"tr","span":{"begin":1376,"end":1425}},{"label":"td","span":{"begin":1376,"end":1382}},{"label":"td","span":{"begin":1383,"end":1404}},{"label":"td","span":{"begin":1405,"end":1425}},{"label":"tr","span":{"begin":1426,"end":1480}},{"label":"td","span":{"begin":1426,"end":1432}},{"label":"td","span":{"begin":1433,"end":1455}},{"label":"td","span":{"begin":1456,"end":1480}},{"label":"tr","span":{"begin":1481,"end":1533}},{"label":"td","span":{"begin":1481,"end":1488}},{"label":"td","span":{"begin":1489,"end":1512}},{"label":"td","span":{"begin":1513,"end":1533}},{"label":"tr","span":{"begin":1534,"end":1591}},{"label":"td","span":{"begin":1534,"end":1541}},{"label":"td","span":{"begin":1542,"end":1566}},{"label":"td","span":{"begin":1567,"end":1591}},{"label":"tr","span":{"begin":1592,"end":1637}},{"label":"td","span":{"begin":1592,"end":1597}},{"label":"td","span":{"begin":15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