PMC:6354430 / 8041-8633
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6354430","sourcedb":"PMC","sourceid":"6354430","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6354430","text":"Viruses\nWNV-NY is strain 3000.0259, isolated in New York in 2000 [42] and passaged twice in C6/36 Aedes albopictus cells to generate an insect cell-derived stock for f.p. inoculations. For i.c. inoculations of WNV-NY, virus strain 3000.0259 was passaged once in Vero cells to generate mammalian cell-derived stock. Attenuated WNV-NS5-E218A was constructed from WNV 3356 strain as described previously [43, 44] and was passaged once in Vero cells, as described previously [45]. Stock titers for all viruses were determined using BHK21 cells for viral plaque assay as previously described [46].","divisions":[{"label":"Title","span":{"begin":0,"end":7}}],"tracks":[{"project":"2_test","denotations":[{"id":"30704498-11585527-63046397","span":{"begin":66,"end":68},"obj":"11585527"},{"id":"30704498-12021317-63046398","span":{"begin":402,"end":404},"obj":"12021317"},{"id":"30704498-17267492-63046399","span":{"begin":406,"end":408},"obj":"17267492"},{"id":"30704498-22589727-63046400","span":{"begin":472,"end":474},"obj":"22589727"},{"id":"30704498-12551996-63046401","span":{"begin":588,"end":590},"obj":"12551996"}],"attributes":[{"subj":"30704498-11585527-63046397","pred":"source","obj":"2_test"},{"subj":"30704498-12021317-63046398","pred":"source","obj":"2_test"},{"subj":"30704498-17267492-63046399","pred":"source","obj":"2_test"},{"subj":"30704498-22589727-63046400","pred":"source","obj":"2_test"},{"subj":"30704498-12551996-63046401","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ec99","default":true}]}]}}