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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6218809","sourcedb":"PMC","sourceid":"6218809","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6218809","text":"The preparation of single-molecule DNA fibers by molecular combing and fiber-FISH was as previously published,3, 33 with the exception of post-hybridization washes, which consisted of three 5-min washes in 2× SSC at 42°C, instead of two 20-min washes in 50% formamide/50% 2× SSC at room temperature.","tracks":[{"project":"2_test","denotations":[{"id":"30388403-25848046-2049230","span":{"begin":110,"end":111},"obj":"25848046"}],"attributes":[{"subj":"30388403-25848046-2049230","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecd2","default":true}]}]}}