PMC:6218809 / 13051-13674 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6218809","sourcedb":"PMC","sourceid":"6218809","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6218809","text":"Routine genotyping included a DUP4 positive control in every experiment (sample HG02554). We distinguished homozygotes by quantification of DUP4 and control band intensity on agarose gels using ImageJ, and calculating the ratio of DUP4:control band intensity for each individual. At low allele frequencies, homozygotes are expected to be rare. After log2 transformation, a cluster of four outliers of high ratio (\u003e2 SD, log2ratio \u003e 1.43) were clearly separated from the 278 other DUP4-positive samples, and these four were classified as homozygotes. The remaining 278 DUP4-positive samples were classified as heterozygotes.","tracks":[]}