PMC:6218809 / 11215-11628 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6218809","sourcedb":"PMC","sourceid":"6218809","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6218809","text":"1 μg genomic DNA was randomly fragmented to a size of 350 bp by shearing, DNA fragments were end polished, A-tailed, and ligated with the NEBNext adaptor for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and the resulting libraries were analyzed for size distribution by an Agilent 2100 Bioanalyzer and quantified using real-time PCR.","tracks":[]}