PMC:6194691 / 81301-82470 JSONTXT

{"project":"MyTest","denotations":[{"id":"30340614-6150484-30706227","span":{"begin":169,"end":172},"obj":"6150484"},{"id":"30340614-3207358-30706228","span":{"begin":228,"end":231},"obj":"3207358"},{"id":"30340614-2062858-30706229","span":{"begin":288,"end":291},"obj":"2062858"},{"id":"30340614-8263045-30706230","span":{"begin":345,"end":348},"obj":"8263045"},{"id":"30340614-9298843-30706231","span":{"begin":401,"end":404},"obj":"9298843"},{"id":"30340614-18219561-30706232","span":{"begin":455,"end":458},"obj":"18219561"},{"id":"30340614-21291474-30706233","span":{"begin":495,"end":498},"obj":"21291474"},{"id":"30340614-21707071-30706234","span":{"begin":538,"end":541},"obj":"21707071"},{"id":"30340614-23650139-30706235","span":{"begin":579,"end":582},"obj":"23650139"},{"id":"30340614-11278779-30706236","span":{"begin":667,"end":670},"obj":"11278779"},{"id":"30340614-25703048-30706237","span":{"begin":738,"end":740},"obj":"25703048"},{"id":"30340614-28954515-30706238","span":{"begin":798,"end":801},"obj":"28954515"},{"id":"30340614-11278779-30706239","span":{"begin":1165,"end":1168},"obj":"11278779"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"Table 2 Expression of GLUT1 at the blood–brain barrier\nSource Methoda Species Expression/pmol mg−1\nLuminal Totalb Abluminal\nRelative to microvessel protein\nDick et al. [303] Cytochalasin B binding Rat, pig 69–80\nKalaria et al. [571] Cytochalasin B binding Human 42\nFarrell and Pardridge [572] Immunogold e.m Rat 12%c 100%c 48%c\nCornford et al. [573] Immunogold e.m Human 48% 100% 18%\nVannucci et al. [574] Cytochalasin B binding Rat 40–125\nKamiie et al. [182] Proteomics Mouse 90\nUchida et al. [184] Proteomics Human 139\nShawahna et al. [176] Proteomics Human 78.5\nHoshi et al. [185] Lysate digestion proteomics Rat 84–98\nRelative to membrane protein\nSimpson et al. [575] Fractionation, cytochalasin B binding Bovine 620 280\nKubo et al. [58] Fractionation, proteomics Porcine 79% 21%\nZhang et al. [188] Proteomics Porcine 300\naFractionation = fractionation of isolated plasma membranes\nbFor immunogold detection, values are percentages of the immunogold particles where the total includes cytoplasmic\ncAntigen for the antibody used by Farrell and Pardridge appears to be partially masked for GLUT1 most markedly in the luminal membrane in bovine endothelial cells [575]"}

{"project":"2_test","denotations":[{"id":"30340614-6150484-30706227","span":{"begin":169,"end":172},"obj":"6150484"},{"id":"30340614-3207358-30706228","span":{"begin":228,"end":231},"obj":"3207358"},{"id":"30340614-2062858-30706229","span":{"begin":288,"end":291},"obj":"2062858"},{"id":"30340614-8263045-30706230","span":{"begin":345,"end":348},"obj":"8263045"},{"id":"30340614-9298843-30706231","span":{"begin":401,"end":404},"obj":"9298843"},{"id":"30340614-18219561-30706232","span":{"begin":455,"end":458},"obj":"18219561"},{"id":"30340614-21291474-30706233","span":{"begin":495,"end":498},"obj":"21291474"},{"id":"30340614-21707071-30706234","span":{"begin":538,"end":541},"obj":"21707071"},{"id":"30340614-23650139-30706235","span":{"begin":579,"end":582},"obj":"23650139"},{"id":"30340614-11278779-30706236","span":{"begin":667,"end":670},"obj":"11278779"},{"id":"30340614-25703048-30706237","span":{"begin":738,"end":740},"obj":"25703048"},{"id":"30340614-28954515-30706238","span":{"begin":798,"end":801},"obj":"28954515"},{"id":"30340614-11278779-30706239","span":{"begin":1165,"end":1168},"obj":"11278779"}],"text":"Table 2 Expression of GLUT1 at the blood–brain barrier\nSource Methoda Species Expression/pmol mg−1\nLuminal Totalb Abluminal\nRelative to microvessel protein\nDick et al. [303] Cytochalasin B binding Rat, pig 69–80\nKalaria et al. [571] Cytochalasin B binding Human 42\nFarrell and Pardridge [572] Immunogold e.m Rat 12%c 100%c 48%c\nCornford et al. [573] Immunogold e.m Human 48% 100% 18%\nVannucci et al. [574] Cytochalasin B binding Rat 40–125\nKamiie et al. [182] Proteomics Mouse 90\nUchida et al. [184] Proteomics Human 139\nShawahna et al. [176] Proteomics Human 78.5\nHoshi et al. [185] Lysate digestion proteomics Rat 84–98\nRelative to membrane protein\nSimpson et al. [575] Fractionation, cytochalasin B binding Bovine 620 280\nKubo et al. [58] Fractionation, proteomics Porcine 79% 21%\nZhang et al. [188] Proteomics Porcine 300\naFractionation = fractionation of isolated plasma membranes\nbFor immunogold detection, values are percentages of the immunogold particles where the total includes cytoplasmic\ncAntigen for the antibody used by Farrell and Pardridge appears to be partially masked for GLUT1 most markedly in the luminal membrane in bovine endothelial cells [575]"}