PMC:6194691 / 223756-224721 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6194691","sourcedb":"PMC","sourceid":"6194691","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6194691","text":"Caution is advisable in the interpretation of flux data using the carrier model for at least three reasons: steady-state data cannot determine all of the rate constants even in the “simple” carrier model (see e.g. [326]); there must be transport across two membranes in series; and there is likely to be interaction between the GLUT1 monomers in the tetramers thought to be present in membranes. Thus it would be unwise to attach mechanistic significance to the values of constants determined by fitting the model to data. However, the forms of the equations relating the fluxes to the concentrations [325–327, 550] remain the simplest available framework capable of describing the transport. Using the constants that are defined below,33 Tinf=T1,maxK2cplasma1+cisfRK1K2+K2cplasma+K1cisf+cplasmacisfKinh34 Teff=T2,maxK1cisf1+cplasmaRK1K2+K2cplasma+K1cisf+cplasmacisfKinhand35 Tnet=T1,maxK2cplasma-cisfK1K2+K2cplasma+K1cisf+cplasmacisfKinh36 withT1,max/K1=T2,max/K2.","divisions":[{"label":"label","span":{"begin":736,"end":738}},{"label":"label","span":{"begin":803,"end":805}},{"label":"label","span":{"begin":873,"end":875}},{"label":"label","span":{"begin":938,"end":940}}],"tracks":[]}