PMC:6172693 / 45647-46232 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6172693","sourcedb":"PMC","sourceid":"6172693","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6172693","text":"Inverted polarity native gels for ADPr detection\nThis simple, non-radioactive method allows visualization of both substrates and products of ADPr reactions. ADPr reactions were performed as described above, except with 2 μg histone peptide per reaction, and in the presence of non-radioactive NAD+. Samples were mixed with TBE Sample Buffer and loaded on 20% TBE gels in TBE Running Buffer. Samples were run with inverted polarity at 200 V for 1 hr. Gels were then fixed for 30 min in 10% glutaraldehyde, washed in H20 for 3 × 10 min, then stained with Imperial Protein Stain for 1 hr.","divisions":[{"label":"Title","span":{"begin":0,"end":48}}],"tracks":[]}