PMC:6145447 / 46818-47785 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/6145447","sourcedb":"PMC","sourceid":"6145447","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6145447","text":"Extended Data Fig. 1 Actin filament nucleators localize to chromosomal DSBs in Xenopus extracts and mammalian cells\na, Enrichment ratios and spectral counts of actin complexes and repair proteins in DSB-containing chromatin by liquid chromatography-mass spectrometry are shown. b, DNA damage-dependent enrichment of actin complexes (+PflMI) following PIKK inhibition (KU55933: ATMi; VE821: ATRi). RPA shown as a marker of DNA damage. c, Quantification actin complexes in chromatin relative to + PflMI samples. (β-actin n=3 independent experiments, Arpc4 n=2 independent experiments, CapZβ n=3 independent experiments). Mean and s.e.m shown. d, Representative images of WASP foci post NCS treatment in MTFs. e, Quantification of WASP foci (P calculated by two-sided Mann-Whitney test; data shown as mean; DMSO n=578 nuclei, NCS n=556 nuclei). f, γH2AX co-localization with WASP foci in MTFs (n = 22 nuclei, r = 0.58 ± 0.021, Pearson). Scale bar is 5 μm or as shown.\nE","divisions":[{"label":"Title","span":{"begin":22,"end":116}}],"tracks":[]}