PMC:5985359 / 29684-30278 JSONTXT

{"project":"2_test","denotations":[{"id":"29526280-19667189-2044799","span":{"begin":345,"end":347},"obj":"19667189"},{"id":"29526280-23511335-2044800","span":{"begin":349,"end":351},"obj":"23511335"}],"text":"A fully 2′-O-methyl-phosohorothioate(2′-O-Me-PS) modified (CAG)7 ASO complimentary to (CUG)n repeats has previously been shown to effectively silence DMPK1 trinucleotide repeat expansion transcripts in a DM1 humanized animal model and human DM1 cell system and to reduce the number of DM1-associated RNA foci in a repeat-length-dependent manner.23, 24 We therefore tested whether transfecting with 2′-O-Me-PS-(CAG)7 ASOs, complimentary to TCF4 CTG18.1-derived transcripts, could induce a similar reduction in CUG-specific RNA foci, a biomarker of CTG18.1-related pathology, in the CEC cultures."}