PMC:5985359 / 16159-16838 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5985359","sourcedb":"PMC","sourceid":"5985359","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5985359","text":"Total RNA was extracted from primary CECs using NucleoSpin RNA XS kit according to manufacturer’s guidelines (Macherey-Nagel). cDNA was reverse-transcribed using a Tetro cDNA synthesis kit (BIOLINE) with an oligo (dT)18 primer mix in accordance with manufacturer’s guidelines. Reverse transcription PCR was performed using intron spanning primers listed in Table S2 using GoTaq Green Master mix (Promega) and standard cycling parameters. The identities of all amplified products were confirmed by Sanger sequencing using standard methodologies. The relative intensities of PCR-amplified products resolved on agarose gels were calculated using Image Lab software package (BioRad).","tracks":[]}