PMC:5944998 / 9432-10949 JSONTXT

{"project":"2_test","denotations":[{"id":"29746533-23835361-90904097","span":{"begin":1185,"end":1186},"obj":"23835361"}],"text":"Plant material and JA treatment\nStrawberry (F. × ananassa cv. Aromas) flowers and fruit were collected at different developmental stages from plants grown in a commercial field at Angol, Araucanía Region, Chile (latitude 37°45’18” S; longitude 72°36’49” W) during three different dates in the 2014 growing season. The owner of the land gave permission to conduct the study on this site. The picked flowers and fruit were transported to the laboratory under refrigerated conditions and classified in six developmental stages corresponding to 0 (flowering, F), 10 (small green, SG), 17 (large green, LG), 20 (white, W), 21 (turning, T), 23 (50% red receptacle, 50%R) and 25 (100% red receptacle, 100%R) days after anthesis (DAA) as previously reported [7].\nOn the other hand, we performed an experiment to verify JA treatment effects on MYC2 and JAZs gene expression during an in vitro fruit ripening system. Peduncles of three fruit at white stage (W) were trimmed to a uniform length of 50 mm and immersed in sterile tubes (50 ml) with autoclaved distilled water containing 88 mM sucrose and 1 mM hydroxyquinoline hemisulfate (HQS) plus 100 μM MeJA according to previously reported [7,9]. The fruit in solution were incubated in a growth chamber under standard fluorescent lights (16 h photoperiod and 40 μmol/m2.s1 light intensity) at 24°C. Fruit sampling was performed at 15 min, 30 min, 1 h and 6 h of MeJA incubation. At each treatment and time, three biological replicates were used for gene expression analysis."}