PMC:5944998 / 8328-9430 JSONTXT

{"project":"2_test","denotations":[{"id":"29746533-25504850-90904092","span":{"begin":150,"end":152},"obj":"25504850"},{"id":"29746533-24170397-90904093","span":{"begin":573,"end":575},"obj":"24170397"},{"id":"29746533-19151095-90904094","span":{"begin":613,"end":615},"obj":"19151095"},{"id":"29746533-15173120-90904095","span":{"begin":688,"end":690},"obj":"15173120"},{"id":"29746533-27131786-90904096","span":{"begin":899,"end":901},"obj":"27131786"}],"text":"Gene structure, protein sequence and phylogenetic analyses\nExon-intron organizations of JAZ and MYC genes were determined by Gene Display Server 2.0 [34] using the information available at Genome database (https://www.ncbi.nlm.nih.gov/genome). F. vesca and Arabidopsis JAZ and MYC TFs sequences were analyzed for structural and functional domains. F. vesca JAZs and MYCs encoding sequences were obtained from F. vesca transcriptome (https://www.ncbi.nlm.nih.gov/refseq) (S1 Table). TIFY and Jas domains were identified through multiple sequence alignment by Clustal Omega [35] and visualized by Jalview software [36]. Logo sequences of JAZ and MYCs domains were obtained using Weblogo 3 [37]. Unrooted phylogenetic trees were built using full-length amino acidic sequences by the neighbor-joining (NJ) method and a bootstrap of 1,000 replicates. Phylogenetic trees were visualized by Evolview tool [38]. We renamed the annotated F. vesca TIFY and MYC sequences according to their homology degree; length, domain location and clustering in phylogenetic tree with the corresponding Arabidopsis sequences."}