PMC:5711939 / 36019-36528 JSONTXT

{"project":"2_test","denotations":[{"id":"29214045-23206248-76397148","span":{"begin":128,"end":130},"obj":"23206248"}],"text":"Effects of AMPs on bacterial cell morphology was investigated using transmission electron microscopy as described by Huws et al.49 Bacterial cultures in mid-log phase were treated with Lynronne-1, Lynronne-2 and Lynronne-3 (at 3× MIC for 1 h) and then fixed with 2.5% (v/v) glutaraldehyde. Cells were post-fixed with 1% osmium tetroxide (w/v), stained with 2% (w/v) uranyl acetate and Reynold’s lead citrate and observed using a JEOL JEM1010 transmission electron microscope (JEOL Ltd, Tokyo, Japan) at 80 kV."}