PMC:5711939 / 31028-31743 JSONTXT

{"project":"2_test","denotations":[{"id":"29214045-8157999-76397142","span":{"begin":113,"end":115},"obj":"8157999"},{"id":"29214045-19883637-76397143","span":{"begin":713,"end":715},"obj":"19883637"}],"text":"Cytotoxic activity of the identified peptides was determined using HUVEC and HEPG2 cells as described previously.41,42 Three dose response parameters 50% growth inhibition (GI50), total growth inhibition (TGI) and 50% lethal concentration (LC50) were calculated for each experimental agent and cellular tumor assay results are presented as the percent cell growth or percent tube length of specific activity. Significant responses were defined when observed cell growth was ≤50% and tube length was ≤70% using the proliferation and tube formation assays respectively. Permeabilization of eukaryotic membrane by Lynronne-1, Lynronne-2 and Lynronne-3 was also investigated using HepG2 cells as described previously.43"}