PMC:5711939 / 25653-26437 JSONTXT

{"project":"2_test","denotations":[{"id":"29214045-25575887-76397126","span":{"begin":113,"end":115},"obj":"25575887"}],"text":"A metagenomic library consisting of 8448 clones from a plant-associated rumen microbiome was prepared previously.15 The clones were transferred gently using multichannel pin replicators (Molecular Devices Ltd, Berkshire UK) onto Luria–Bertani agar plates containing a lawn (500 µl) of S. aureus (MSSA) RN4220, E. coli K12, Salmonella enterica serovar Typhimurium SL1344, E. faecalis JH2-2 and L. monocytogenes NCTC 11994 serovar 4b (OD600 = 1) using sterile pin replicators. Plates containing these pathogens were incubated at 37 °C (or 30 °C for L. monocytogenes) for 24 h, and clearing zones around the fosmid clones were considered as indicative of clones containing gene inserts coding for antimicrobial compounds. Assays were performed in sextuplicates to ensure reproducibility."}