PMC:5623723 / 33654-35572 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5623723","sourcedb":"PMC","sourceid":"5623723","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5623723","text":"Neuroprotective Effects of LBP Was also Mediated by Activating NR2A-Akt-CREB Pathways\nNext, we investigated whether LBP-induced neuroprotection against OGD was also mediated by the NR2A-Akt-CREB pathway. Our findings demonstrated NR2A expression was maintained at normal levels with 2 h of OGD exposure before it decreased to 66.5 ± 11.4% at 4 h OGD and 33.5 ± 2.8% at 8 h OGD, respectively (both p \u003c 0.01 vs. control, Figure 6A). Expression of pAkt dramatically decreased from OGD for 30 min to OGD for 8 h (59.5% ± 7.1, 55.9% ± 7.1, 57.5% ± 9.6, 53.6% ± 4.6 and 52.9% ± 8.4, respectively; all p \u003c 0.01 vs. control). Expression of pCREB remained unchanged with 1 h OGD, but dramatically decreased to 50.8% ± 4.6 at 2 h OGD, 46.2% ± 7.4 at 4 h OGD and 32.1% ± 6.6 at 8 h OGD (all p \u003c 0.01 vs. control). We further investigated the effects of LBP on the expression of major proteins related NR2A signaling. At 1 h OGD exposure, we observed no differences in expression of NR2A, pAkt or pCREB between the OGD group and OGD+LBP group (p \u003e 0.05, Figure 6B). However, at 4 h OGD exposure, LBP significantly reversed the decrease in expression of NR2A (from 58.6% ± 0.7 to 93.8% ± 2.0), pAkt (from 45.7 ± 2.4% to 85.9 ± 2.9%) and pCREB (from 29.5% ± 3.2 to 88.5% ± 3.4), respectively (all p \u003c 0.01), when compared with the OGD group (Figure 6C). LBP upregulated the NR2A and pCREB expression to the control level (p \u003e 0.05 vs. control), although expression of pAkt remained lower than the control (p \u003c 0.05 vs. control).\nFigure 6 Effects of LBP on the expression of NR2A, pAkt and pCREB proteins after exposed 4 h OGD. (A) Expression of these proteins at different OGD duration. (B) When exposed to OGD for 1 h, LBP did not influence expression of NR2A, pAkt and pCREB proteins. However, LBP treatment markedly inhibited the decrease in these protein levels after neurons were exposed to 4 h OGD (C; *P \u003c 0.05; **P \u003c 0.01).","divisions":[{"label":"title","span":{"begin":0,"end":85}},{"label":"p","span":{"begin":86,"end":1514}},{"label":"label","span":{"begin":1515,"end":1523}}],"tracks":[]}