PMC:5623723 / 15861-16502 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5623723","sourcedb":"PMC","sourceid":"5623723","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5623723","text":"Neural viability was measured using a MTT reduction kit (Sigma, USA). Briefly, primary cultured neurons were exposed to designated periods of OGD. Twenty-four hours after the reperfusion, neurons were incubated with 500 μg/ml MTT in culture medium at 37°C for 4 h. Next, the medium was removed, and 100 μL DMSO was added into each well of 96 well multiplates to dissolve the crystals. The absorbance at 550 nm was measured with a microplate reader (PerkinElmer, USA). Neural death was determined by measuring the concentration of LDH released from the damaged cells with a LDH assay kit (Dojindo, Japan) following manufacturer’s instruction.","tracks":[]}