PMC:5623723 / 14629-15303 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5623723","sourcedb":"PMC","sourceid":"5623723","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5623723","text":"After cells were cultured in wells or dishes for 2 weeks, the culture medium was removed and the cells washed three times with deoxygenated (bubbled with N2 to remove the residual O2 for 30 min) and glucose-free BSS (consisting of 116 NaCl, 5.4 KCl, 0.8 MgSO4, 1.0 NaH2PO4, 26.2 NaHCO3, 1.8 CaCl2, all in mM). Then, the wells or dishes were placed in an anaerobic chamber (Coy Laboratory, USA) with 5% CO2-95% N2 at 37°C for a designated period of OGD. OGD was terminated by replacement of normal culture medium. Cells were placed back in a standard incubator for 24 h before being analyzed. A control group was cultured with normal culture medium in the standard incubator.","tracks":[]}